PMID- 16321322
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20051202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 85
IP  - 37
DP  - 2005 Sep 28
TI  - [Regulation of hexokinase activity by protein kinase C in peritoneal mesothelium 
      cell].
PG  - 2619-22
AB  - OBJECTIVE: To investigate the effect of protein kinase C (PKC) on the activity of 
      hexokinase (HK) in rat peritoneal mesothelium cell (PMC) and the functional 
      consequences in glucose uptake. METHODS: omentum obtained from albino male rats 
      of the Sprague Dawley strain, PMC were isolated and subculture by enzymatic 
      disaggregation. The antibody to cytokeratin was used for identified by 
      immunofluorescence technique. After treated with PKC activator--phorbol esters 
      (PMA and PDD) and classical PKC (cPKC) inhibitor--Go6976, total HK activity in 
      PMC were measured by a standard G6PDH-coupled assay, glucose disappearance in the 
      culture medium were tested as glucose net utilization by colorimetric method. 
      RESULTS: The PMC were polygonal when confluent and reacted positively for 
      cytokeratin. PMA induced HK activity in a time and dose-dependent manner and 
      increased net glucose utilization. At concentration of 0.01, 0.1 and 1 micromol/L 
      PMA for 24 h, the increase in HK activity were 30.7%, 59.9% and 99.1%, 
      respectively above the level in the control (P < 0.05). After treated with 1 
      micromol/L PMA for 6 h, the HK activity of PMC began to up-regulation, the peak 
      reached at 24 h (21.3% vs 100.4%, P < 0.05), and accompanied by increase in net 
      glucose utilization (2 mmol/L, P < 0.05). The expression of HK induced by 0.1 
      micromol/L PDD were similar to 1 micromol/L PMA, 4 alpha-PDD whose structure 
      resemble to PDD didn't have effect on HK. Go6976 blocked the activity of HK 
      induced by PMA. After incubated with Go6976 at concentration of 10, 20, 30, 40 
      and 50 micromol/L for 30 min, PMC were cultured in 1 micromol/L PMA for 24 h, the 
      decrease in HK activity were 14.6%, 26.8%, 34.2%, 44.2% and 54.9% (P < 0.05). 
      CONCLUSIONS: Elevated HK activity by PKC was accompanied with corresponding 
      increase in net glucose utilization, which might take part in the accelerated 
      glucose uptake after long-term peritoneal dialysis. The blocking of HK activity 
      by cPKC inhibitor might be an effective method for increasing ultrafiltration in 
      peritoneal dialysis.
FAU - Fan, Yi
AU  - Fan Y
AD  - Department of Nephrology, First Clinical College, China Medical University, 
      Shenyang 110001, China.
FAU - Ma, Jian-fei
AU  - Ma JF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Epithelium/*metabolism
MH  - Hexokinase/*metabolism
MH  - Male
MH  - Peritoneum/*cytology/metabolism
MH  - Protein Kinase C/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/12/03 09:00
MHDA- 2013/06/26 06:00
CRDT- 2005/12/03 09:00
PHST- 2005/12/03 09:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
PHST- 2005/12/03 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2005 Sep 28;85(37):2619-22.