PMID- 16323574
OWN - NLM
STAT- MEDLINE
DCOM- 20060203
LR  - 20170214
IS  - 0960-3271 (Print)
IS  - 0960-3271 (Linking)
VI  - 24
IP  - 11
DP  - 2005 Nov
TI  - An in vitro study of Hoechst 33342 redistribution and its effects on cell 
      viability.
PG  - 573-80
AB  - Although Hoechst 33342 (H342) is frequently used to label donor cells in cell 
      transplantation research, it has been noted that it might secondarily label the 
      host cells. Furthermore, its potential toxicity leading to cell death has been 
      described. We studied the time course of H342 redistribution from the primary 
      labeled rat bone marrow stromal cells (rBMSC) into the non-labeled rBMSC 
      population over 7 days in culture; we evaluated the nuclear H342 fluorescence 
      intensity as a possible criterion for distinguishing the primary from the 
      secondary labeled cells, and determined the viability of rBMSC after an overnight 
      incubation in 1 microg/mL of H342. H342 labeled >50% of the initially non-labeled 
      cells within the first 6 hours and almost 90% within a week. Nuclear fluorescence 
      intensity was a reliable criterion for distinguishing primary and secondary 
      labeled cells within the first 24 hours, but less so at later time points. The 
      percentage of either apoptotic or necrotic cells did not rise acutely after the 
      overnight incubation in 1 microg/mL of H342. Although a 12-hour incubation of 
      rBMSC in 1 microg/mL of H342 did not cause acute cell death, H342 rapidly and 
      extensively redistributed into non-labeled cells, which makes H342 a relatively 
      unsuitable marker for cell transplantation research.
FAU - Mohorko, N
AU  - Mohorko N
AD  - LNPR, Faculty of Medicine, Institute of Pathophysiology, Zaloska, University of 
      Ljubljana, Slovenia. nina.mohorko@mf.uni-lj.si
FAU - Kregar-Velikonja, N
AU  - Kregar-Velikonja N
FAU - Repovs, G
AU  - Repovs G
FAU - Gorensek, M
AU  - Gorensek M
FAU - Bresjanac, M
AU  - Bresjanac M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Benzimidazoles)
RN  - 0 (Fluorescent Dyes)
RN  - P976261J69 (bisbenzimide ethoxide trihydrochloride)
SB  - IM
MH  - Animals
MH  - Area Under Curve
MH  - Benzimidazoles/metabolism/*pharmacology
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Bone Marrow Transplantation/methods
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Fluorescent Dyes/metabolism/*pharmacology
MH  - ROC Curve
MH  - Rats
MH  - Rats, Wistar
MH  - Staining and Labeling
MH  - Time Factors
EDAT- 2005/12/06 09:00
MHDA- 2006/02/04 09:00
CRDT- 2005/12/06 09:00
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/02/04 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - 10.1191/0960327105ht570oa [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2005 Nov;24(11):573-80. doi: 10.1191/0960327105ht570oa.