PMID- 16324192
OWN - NLM
STAT- MEDLINE
DCOM- 20060126
LR  - 20181203
IS  - 0309-0167 (Print)
IS  - 0309-0167 (Linking)
VI  - 47
IP  - 6
DP  - 2005 Dec
TI  - Demonstration of EGFR gene copy loss in colorectal carcinomas by fluorescence in 
      situ hybridization (FISH): a surrogate marker for sensitivity to specific 
      anti-EGFR therapy?
PG  - 560-4
AB  - AIMS: To investigate EGFR gene copy number heterogeneity in colorectal carcinomas 
      compared with copy number of chromosome 7 and immunohistochemical expression of 
      the EGFR protein. METHODS AND RESULTS: Fluorescence in situ hybridization of the 
      EGFR gene and CEP7 was carried out on paraffin-embedded material from 48 rectal 
      carcinomas combined with immunohistochemical detection of EGFR with a polymer 
      detection kit. EGFR gene copy number had a range of 1.4-7.3 with a mean of 2.5. 
      CEP7 copy number had a range of 1.5-6.1 with a mean of 2.5. The EGFR gene/CEP7 
      ratio ranged from 0.4 to 1.5 with a mean of 0.96. Most cases had a balanced EGFR 
      gene/CEP7 ratio (37 cases = 77%). Copy gain was found in seven cases (15%) with a 
      ratio of up to 1.5, consistent with gain of one EGFR gene copy in one chromosome. 
      Copy loss was found in four cases (8%). All cases with EGFR gene copy loss were 
      immunohistochemically positive. CONCLUSIONS: Demonstration of EGFR gene copy loss 
      might be a surrogate marker for EGFR mutation/deletion and could be used in a 
      routine setting in pathology departments. Further studies are needed to determine 
      whether this may be used to select patients that might benefit from specific 
      anti-EGFR therapy.
FAU - Sauer, T
AU  - Sauer T
AD  - Department of Pathology, Ullevaal University Hospital, Oslo, Norway. 
      torill.sauer@medisin.uio.no
FAU - Guren, M G
AU  - Guren MG
FAU - Noren, T
AU  - Noren T
FAU - Dueland, S
AU  - Dueland S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Carcinoma/*genetics/metabolism/pathology
MH  - Chromosomes, Human, Pair 7
MH  - Colorectal Neoplasms/*genetics/metabolism/pathology/therapy
MH  - ErbB Receptors/*genetics/metabolism
MH  - *Gene Dosage
MH  - Gene Expression
MH  - *Genes, erbB-1
MH  - Genes, erbB-2
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Sensitivity and Specificity
EDAT- 2005/12/06 09:00
MHDA- 2006/01/27 09:00
CRDT- 2005/12/06 09:00
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/01/27 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - HIS2252 [pii]
AID - 10.1111/j.1365-2559.2005.02252.x [doi]
PST - ppublish
SO  - Histopathology. 2005 Dec;47(6):560-4. doi: 10.1111/j.1365-2559.2005.02252.x.