PMID- 16325784
OWN - NLM
STAT- MEDLINE
DCOM- 20060215
LR  - 20061115
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1066
IP  - 1-2
DP  - 2005 Dec 20
TI  - Systematic analysis of axonal damage and inflammatory response in different white 
      matter tracts of acutely injured rat spinal cord.
PG  - 57-70
AB  - The mechanisms of white matter (WM) damage during secondary degeneration are a 
      fundamental issue in the pathophysiology of central nervous system (CNS) 
      diseases. Our main goal was to describe the pattern of an acute inflammatory 
      response and secondary damage to axons in different WM tracts of acutely injured 
      rat spinal cord. Adult rats were deeply anesthetized and injected with 20 nmol of 
      NMDA into the spinal cord ventral horn on T7. Animals were perfused after 
      survival times of 1 day, 3 days and 7 days. Ten micrometer sections were 
      submitted to immunocytochemical analysis for activated macrophages/microglia, 
      neutrophils and damaged axons. There were inflammatory response and progressive 
      tissue destruction of ventral WM (VWM) with formation of microcysts in both VWM 
      and lateral WM (LWM). In the VWM, the number of beta-amyloid precursor protein 
      (beta-APP) end-bulbs increased from 1 day with a peak at 3 days, decreasing by 7 
      days following the injection. APP end-bulbs were present in the dorsal WM (DWM) 
      at 3 days survival time but were not in the LWM. Electron microscopic analysis 
      revealed different degrees of myelin disruption and axonal pathology in the 
      vacuolated WM up to 14 mm along the rostrocaudal axis. Quantitative analysis 
      revealed a significant loss of medium and large axons (P < 0.05), but not of 
      small axons (P > 0.05). Our results suggest that bystander axonal damage and 
      myelin vacuolation are important secondary component of the pathology of WM 
      tracts following rat SCI. Further studies are needed to understand the mechanisms 
      of these pathological events.
FAU - Gomes-Leal, W
AU  - Gomes-Leal W
AD  - Laboratorio de Neuroanatomia Funcional, Departamento de Morfologia, Centro de 
      Ciencias Biologicas, Universidade Federal do Para, Rua Augusto Correa S/N, Campus 
      do Guama, CEP:66075-900. Belem-Para, Brasil. walaceleal@yahoo.com.br
FAU - Corkill, D J
AU  - Corkill DJ
FAU - Picanco-Diniz, C W
AU  - Picanco-Diniz CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051202
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Neurotoxins)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Animals
MH  - Axons/*pathology
MH  - Cell Count
MH  - Excitatory Amino Acid Agonists/toxicity
MH  - Immunohistochemistry
MH  - Inflammation/*pathology
MH  - Macrophages/pathology
MH  - Male
MH  - Microglia/pathology
MH  - Microscopy, Electron, Transmission
MH  - N-Methylaspartate/toxicity
MH  - Neurotoxins/toxicity
MH  - Neutrophils/pathology
MH  - Perfusion
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord Injuries/*pathology
EDAT- 2005/12/06 09:00
MHDA- 2006/02/16 09:00
CRDT- 2005/12/06 09:00
PHST- 2005/05/25 00:00 [received]
PHST- 2005/10/05 00:00 [revised]
PHST- 2005/10/11 00:00 [accepted]
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/02/16 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - S0006-8993(05)01494-0 [pii]
AID - 10.1016/j.brainres.2005.10.069 [doi]
PST - ppublish
SO  - Brain Res. 2005 Dec 20;1066(1-2):57-70. doi: 10.1016/j.brainres.2005.10.069. Epub 
      2005 Dec 2.