PMID- 16325850
OWN - NLM
STAT- MEDLINE
DCOM- 20060227
LR  - 20061115
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 355
IP  - 4
DP  - 2006 Jan 27
TI  - Ligand binding energy and enzyme efficiency from reductions in protein dynamics.
PG  - 760-7
AB  - Tetrameric rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (GAPDH; EC 
      1.2.1.12) binds successively four molecules of its cofactor (NAD+) with 
      affinities of ca 10(11) M(-1), 10(9) M(-1), 10(7) M(-1), and 10(5) M(-1). The 
      reduction in the dynamics of the protein is greatest upon binding the first NAD+ 
      molecule. Smaller reductions then occur upon binding the second and third NAD+ 
      molecules, and the fourth NAD+ molecule binds without dynamic change. Reduction 
      of the GAPDH dynamics, with consequent improvements in its internal bonding, can 
      account for the increase in NAD+ binding affinity from 10(5) M(-1) to 10(11) 
      M(-1). Evidence is provided that comparable fractions of the binding energy of 
      other ligands, and of the catalytic efficiency of enzymes, may be derived in the 
      same way.
FAU - Williams, Dudley H
AU  - Williams DH
AD  - Department of Chemistry, Lensfield Road, Cambridge CB2 1EW, UK. dhw1@cam.ac.uk
FAU - Zhou, Min
AU  - Zhou M
FAU - Stephens, Elaine
AU  - Stephens E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051122
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Ligands)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Catalysis
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*chemistry/*metabolism
MH  - Ligands
MH  - Models, Biological
MH  - Muscles/chemistry/metabolism
MH  - Oxidation-Reduction
MH  - Protein Binding
MH  - Rabbits
MH  - Thermodynamics
EDAT- 2005/12/06 09:00
MHDA- 2006/02/28 09:00
CRDT- 2005/12/06 09:00
PHST- 2005/09/02 00:00 [received]
PHST- 2005/10/20 00:00 [revised]
PHST- 2005/11/05 00:00 [accepted]
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/02/28 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - S0022-2836(05)01401-4 [pii]
AID - 10.1016/j.jmb.2005.11.015 [doi]
PST - ppublish
SO  - J Mol Biol. 2006 Jan 27;355(4):760-7. doi: 10.1016/j.jmb.2005.11.015. Epub 2005 
      Nov 22.