PMID- 16327270
OWN - NLM
STAT- MEDLINE
DCOM- 20060418
LR  - 20060110
IS  - 0301-1569 (Print)
IS  - 0301-1569 (Linking)
VI  - 67
IP  - 6
DP  - 2005
TI  - Cyclin D1 protein tissue detection in laryngeal cancer.
PG  - 319-25
AB  - Cyclin D1 (CCND1) is a set of periodic regulatory proteins that is believed to 
      govern cell cycle transit from G1 into S phase. Overexpression of CCND1 leads to 
      abnormal cellular proliferation which underlies processes of tumorigenesis; CCND1 
      can thus function as a cooperative oncogene in cell transformation. In the 
      present study we investigate the immunohistochemical expression of CCND1 in a 
      well-documented series of 58 laryngeal squamous cell carcinomas (LSCC) and search 
      for statistical associations between CCND1 index and various clinicopathological 
      parameters including several immunomarkers' expression as well as patients' 
      disease-free survival. Tissue sections from archival paraffin blocks were stained 
      using the avidin-biotin-peroxidase complex method; the H-295 rabbit polyclonal 
      antibody was applied at dilution of 1:150. The percentage of CCND1 immunoreactive 
      tumor cells for each tumor was counted by an image analysis system. CCND1 
      staining was confined to cell nuclei and, in the examined samples, ranged from 
      undetectable (i.e. 0% of tumor cells, n = 6) to the majority of tumor cells (i.e. 
      89% of tumor cells) with mean value: 15.73%. In tumor adjacent, non invasive 
      lesions, strong CCND1 staining was noticed in areas with cellular atypia. In 
      cases with nodal metastases, no change in CCND1 expression in the nodal 
      metastases compared with the primary tumors was observed. p53 protein 
      accumulation in malignant cells was positively linked with CCND1 index 
      (Mann-Whitney U: 205.5, p = 0.034). CCND1 expression appears to be an early event 
      in processes of tumorigenesis and tumor progression in some LSCC. Apart from p53 
      protein accumulation, CCND1 immunohistochemical expression does not seem to 
      correlate with nodal metastasis, disease recurrence or any other 
      clinicopathological prognostic indicator.
FAU - Segas, John V
AU  - Segas JV
AD  - Department of Otolaryngology, School of Medicine, National and Kapodistrian 
      University of Athens, Greece. jsegas@med.uoa.gr
FAU - Lazaris, Andreas C
AU  - Lazaris AC
FAU - Nikolopoulos, Thomas P
AU  - Nikolopoulos TP
FAU - Kavantzas, Nikolaos G
AU  - Kavantzas NG
FAU - Lendari, Irene E
AU  - Lendari IE
FAU - Tzagkaroulakis, Antonios M
AU  - Tzagkaroulakis AM
FAU - Patsouris, Efstratios S
AU  - Patsouris ES
FAU - Ferekidis, Eleftherios A
AU  - Ferekidis EA
LA  - eng
PT  - Journal Article
DEP - 20051201
PL  - Switzerland
TA  - ORL J Otorhinolaryngol Relat Spec
JT  - ORL; journal for oto-rhino-laryngology and its related specialties
JID - 0334721
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/*metabolism/pathology
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Cyclin D1/*genetics/*metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Recurrence
EDAT- 2005/12/06 09:00
MHDA- 2006/04/19 09:00
CRDT- 2005/12/06 09:00
PHST- 2004/01/29 00:00 [received]
PHST- 2004/10/01 00:00 [accepted]
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/04/19 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - 90041 [pii]
AID - 10.1159/000090041 [doi]
PST - ppublish
SO  - ORL J Otorhinolaryngol Relat Spec. 2005;67(6):319-25. doi: 10.1159/000090041. 
      Epub 2005 Dec 1.