PMID- 16328410
OWN - NLM
STAT- MEDLINE
DCOM- 20060822
LR  - 20181113
IS  - 0937-4477 (Print)
IS  - 0937-4477 (Linking)
VI  - 262
IP  - 12
DP  - 2005 Dec
TI  - Viable head and neck tumor spheroids stimulate in vitro autologous monocyte MCP-1 
      secretion through soluble substances and CD14/lectin-like receptors.
PG  - 953-60
AB  - Biopsies from carcinoma tissue and benign control mucosa from head and neck 
      squamous cell carcinoma (HNSCC) patients were used to establish fragment 
      (F)-spheroids in vitro. We have previously shown that autologous monocytes 
      co-cultured with F-spheroids in vitro augment their secretion of monocyte 
      chemotactic protein-1 (MCP-1). Presently, the aims of the present work were to 
      study whether the metabolic activity, secreted products and/or specific 
      receptor/ligand on the surface of the F-spheroids and monocytes are necessary for 
      stimulation of the monocyte MCP-1 secretion upon F-spheroid co-culture. 
      Actinomycin D (1 mug/ml for 24 h) pre-treatment of the F-spheroids abolished the 
      monocyte MCP-1 co-culture response. Co-culture of monocytes and F-spheroids 
      separated by a semi-permeable membrane showed a decreased, but still present, 
      monocyte MCP-1 co-culture response. Conditioned medium from F-spheroids 
      stimulated allogenous monocytes to secrete MCP-1. The addition of glucose or 
      galactose, but not mannose, to co-cultures partially inhibited the monocyte MCP-1 
      co-culture response. The addition of anti-CD14 antibody diminished the MCP-1 
      co-culture response. In conclusion, the monocyte MCP-1 co-culture response is 
      dependent on metabolically active spheroids, secreted stimuli, and is augmented 
      by direct contact with F-spheroids, possibly via lectin-like receptors and the 
      CD14 receptor.
FAU - Olsnes, Carla
AU  - Olsnes C
AD  - Department of Otolaryngology and Head and Neck Surgery, Haukeland University 
      Hospital, Bergen, Norway.
FAU - Heimdal, John-Helge
AU  - Heimdal JH
FAU - Kross, Kenneth W
AU  - Kross KW
FAU - Olofsson, Jan
AU  - Olofsson J
FAU - Aarstad, Hans Jorgen
AU  - Aarstad HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051119
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (Autoantibodies)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, Mitogen)
RN  - IY9XDZ35W2 (Glucose)
RN  - PHA4727WTP (Mannose)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Autoantibodies/immunology
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Chemokine CCL2/*biosynthesis/metabolism
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Galactose/pharmacology
MH  - Glucose/pharmacology
MH  - Head and Neck Neoplasms/*metabolism/pathology
MH  - Humans
MH  - Ligands
MH  - Lipopolysaccharide Receptors/immunology/*pharmacology
MH  - Mannose/pharmacology
MH  - Monocytes/*metabolism
MH  - Receptors, Mitogen/*metabolism
MH  - Spheroids, Cellular/*metabolism/pathology
MH  - Tumor Cells, Cultured
EDAT- 2005/12/06 09:00
MHDA- 2006/08/23 09:00
CRDT- 2005/12/06 09:00
PHST- 2004/09/17 00:00 [received]
PHST- 2004/11/18 00:00 [accepted]
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/08/23 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - 10.1007/s00405-004-0902-1 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2005 Dec;262(12):953-60. doi: 
      10.1007/s00405-004-0902-1. Epub 2005 Nov 19.