PMID- 16328624
OWN - NLM
STAT- MEDLINE
DCOM- 20060126
LR  - 20131121
IS  - 0090-4341 (Print)
IS  - 0090-4341 (Linking)
VI  - 50
IP  - 1
DP  - 2006 Jan
TI  - Induction of CYP1A mRNA in Carp (Cyprinus carpio) from the Kalamazoo River 
      polychlorinated biphenyl-contaminated superfund site and in a laboratory study.
PG  - 14-22
AB  - The Kalamazoo River Superfund site in Michigan is contaminated with 
      polychlorinated biphenyls (PCBs), which were heavily discharged into the river 
      from several paper companies as part of the deinking process in the 1950s through 
      1970s. We characterized biomarkers of chronic PCB exposure in a resident fish 
      population using real-time reverse transcriptase-polymerase chain reaction to 
      examine mRNA expression levels of multiple genes in carp (Cyprinus carpio) liver 
      from PCB contaminated and reference sites in the Kalamazoo River. We also 
      measured these same genes in juvenile carp exposed to dietary PCBs for 4 months. 
      Kalamazoo River carp had significantly increased levels of cytochrome P450 1A 
      (CYP1A) mRNA as did carp fed PCBs in the laboratory. No significant mRNA 
      upregulation occurred in the specific oxidative stress genes 
      (gamma-glutamylcysteine synthetase and magnesium superoxide dismutase) and 
      metabolic genes (phosphoenolpyruvate carboxykinase and nucleolin) examined. These 
      data are consistent with the idea that carp from the Kalamazoo River Superfund 
      Site are responding to PCB exposure via upregulation of CYP1A independent of 
      activation of the oxidative stress response genes normally thought to be 
      co-regulated with CYP1A.
FAU - Fisher, M A
AU  - Fisher MA
AD  - Environmental Institute, Western Michigan University, 1903 West Michigan Avenue, 
      Kalamazoo, Michigan 49008, USA. marla_fisher@ncsu.edu
FAU - Mehne, C
AU  - Mehne C
FAU - Means, J C
AU  - Means JC
FAU - Ide, C F
AU  - Ide CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20051115
PL  - United States
TA  - Arch Environ Contam Toxicol
JT  - Archives of environmental contamination and toxicology
JID - 0357245
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Carps/genetics/growth & development/*metabolism
MH  - Cytochrome P-450 CYP1A1/*biosynthesis/genetics
MH  - Enzyme Induction
MH  - *Gene Expression Regulation, Developmental
MH  - Laboratories
MH  - Liver/metabolism
MH  - Michigan
MH  - Oxidative Stress
MH  - Polychlorinated Biphenyls/metabolism/*toxicity
MH  - Proteins/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rivers
EDAT- 2005/12/06 09:00
MHDA- 2006/01/27 09:00
CRDT- 2005/12/06 09:00
PHST- 2004/10/18 00:00 [received]
PHST- 2005/05/14 00:00 [accepted]
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/01/27 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - 10.1007/s00244-004-0171-4 [doi]
PST - ppublish
SO  - Arch Environ Contam Toxicol. 2006 Jan;50(1):14-22. doi: 
      10.1007/s00244-004-0171-4. Epub 2005 Nov 15.