PMID- 16329118
OWN - NLM
STAT- MEDLINE
DCOM- 20060413
LR  - 20071115
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 108
IP  - 1
DP  - 2006 Feb 25
TI  - The feasibility of gene expression profiling generated in fine-needle aspiration 
      specimens from patients with follicular lymphoma and diffuse large B-cell 
      lymphoma.
PG  - 10-20
AB  - Lymphoma of germinal center cell (GC) origin generally is an indolent malignancy 
      that transforms progressively into a more aggressive disease. According to the 
      World Health Organization classification, lymphomas of follicular center cell 
      origin are classified as either large B-cell lymphoma (LBCL) or follicular 
      lymphoma (FL). The authors tested the feasibility of performing gene expression 
      profiling using amplified RNA from fine-needle aspirates (FNA) obtained from 
      lymph nodes. Twenty-four samples from patients with a diagnosis of FL or LBCL 
      were obtained after Institutional Review Board-approved informed consent was 
      obtained. The diagnoses were confirmed by 2 pathologists and were classified into 
      2 groups (10 LBCL samples and 14 FL samples) by using conventional morphology and 
      immunophenotyping. One hundred nanograms of total RNA were subjected to 2 cycles 
      of standard, double-stranded complementary DNA synthesis and in vitro 
      transcription for target amplification using a small-sample target-labeling 
      protocol. The biotinylated cRNA from each sample was hybridized to gene chips. 
      Gene expression profiling results were analyzed first by principal-component 
      analysis (PCA) by using a list of 146 probe sets that represented 62 genes that 
      are characteristic of an activated B-cell (ABC) signature or a GC signature. The 
      analysis identified 5 LBCL samples with an ABC cell signature. Using a list of 
      207 probe sets that represented 113 genes involved in FL transformation, PCA 
      analysis identified 2 overlapping clusters corresponding to FL and GC-diffuse 
      LBCL. To improve this classification further, the authors generated a list of 72 
      genes that were expressed differentially between FL and GC-LBCL. Using this list 
      of genes, PCA analysis demonstrated a clear separation between FL and GC-LBCL. 
      However, five FL samples clustered as an intermediate group between FL and 
      GC-DLBCL. These samples were characterized morphologically by a mixed cell 
      pattern with relatively fewer large, noncleaved lymphocytes and more small, 
      cleaved lymphocytes. The results support the feasibility of FNA-based 
      transcription profiles in patients with FL or LBCL, which, in combination with 
      morphology and immunophenotyping, can help in the subtyping of these entities.
CI  - (c) 2006 American Cancer Society.
FAU - Goy, Andre
AU  - Goy A
AD  - Department of Lymphoma and Myeloma, The University of Texas M. D. Anderson Cancer 
      Center, Houston, Texas, USA. agoy@humed.com
FAU - Stewart, John
AU  - Stewart J
FAU - Barkoh, Bedia A
AU  - Barkoh BA
FAU - Remache, Yvonne K
AU  - Remache YK
FAU - Katz, Ruth
AU  - Katz R
FAU - Sneige, Nour
AU  - Sneige N
FAU - Gilles, Frederic
AU  - Gilles F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
CIN - Cancer. 2007 Jun 25;111(3):200-1; author reply 201-2. PMID: 17520702
MH  - *Biopsy, Fine-Needle
MH  - Gene Expression
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Immunophenotyping
MH  - Lymph Nodes/surgery
MH  - Lymphoma, B-Cell/*genetics
MH  - Lymphoma, Follicular/*genetics
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Principal Component Analysis
EDAT- 2005/12/06 09:00
MHDA- 2006/04/14 09:00
CRDT- 2005/12/06 09:00
PHST- 2005/12/06 09:00 [pubmed]
PHST- 2006/04/14 09:00 [medline]
PHST- 2005/12/06 09:00 [entrez]
AID - 10.1002/cncr.21500 [doi]
PST - ppublish
SO  - Cancer. 2006 Feb 25;108(1):10-20. doi: 10.1002/cncr.21500.