PMID- 16329884
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20191210
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 51
IP  - 3
DP  - 2005 Feb 1
TI  - Biodegradation efficiency of functionally important populations selected for 
      bioaugmentation in phenol- and oil-polluted area.
PG  - 363-73
AB  - Denaturing gradient gel electrophoresis of amplified fragments of genes coding 
      for 16S rRNA and for the largest subunit of multicomponent phenol hydroxylase 
      (LmPH) was used to monitor the behaviour and relative abundance of mixed 
      phenol-degrading bacterial populations (Pseudomonas mendocina PC1, P. fluorescens 
      strains PC18, PC20 and PC24) during degradation of phenolic compounds in phenolic 
      leachate- and oil-amended microcosms. The analysis indicated that specific 
      bacterial populations were selected in each microcosm. The naphthalene-degrading 
      strain PC20 was the dominant degrader in oil-amended microcosms and strain PC1 in 
      phenolic leachate microcosms. Strain PC20 was not detectable after cultivation in 
      phenolic leachate microcosms. Mixed bacterial populations in oil-amended 
      microcosms aggregated and formed clumps, whereas the same bacteria had a 
      planktonic mode of growth in phenolic leachate microcosms. Colony hybridisation 
      data with catabolic gene specific probes indicated that, in leachate microcosms, 
      the relative proportions of bacteria having meta (PC1) and ortho (PC24) pathways 
      for degradation of phenol and p-cresol changed alternately. The shifts in the 
      composition of mixed population indicated that different pathways of metabolism 
      of aromatic compounds dominated and that this process is an optimised response to 
      the contaminants present in microcosms.
FAU - Heinaru, Eeva
AU  - Heinaru E
AD  - Department of Genetics, Institute of Molecular and Cell Biology, University of 
      Tartu, Riia 23, 51010 Tartu, Estonia. eheinaru@ebc.ee
FAU - Merimaa, Merike
AU  - Merimaa M
FAU - Viggor, Signe
AU  - Viggor S
FAU - Lehiste, Merit
AU  - Lehiste M
FAU - Leito, Ivo
AU  - Leito I
FAU - Truu, Jaak
AU  - Truu J
FAU - Heinaru, Ain
AU  - Heinaru A
LA  - eng
SI  - GENBANK/AF228366
SI  - GENBANK/AF228367
SI  - GENBANK/AF232713
SI  - GENBANK/AF538264
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041022
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Petroleum)
RN  - 0 (Phenols)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.13.7 (phenol 2-monooxygenase)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Culture Media
MH  - DNA, Bacterial/analysis
MH  - DNA, Ribosomal/analysis
MH  - *Ecosystem
MH  - Electrophoresis/methods
MH  - Mixed Function Oxygenases/genetics
MH  - Molecular Sequence Data
MH  - Petroleum/*metabolism
MH  - Phenols/*metabolism
MH  - Plankton/classification/genetics/growth & development/metabolism
MH  - Polymerase Chain Reaction
MH  - Pseudomonas/classification/genetics/*growth & development/metabolism
MH  - Pseudomonas fluorescens/classification/genetics/*growth & development/metabolism
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2005/12/07 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/12/07 09:00
PHST- 2004/05/26 00:00 [received]
PHST- 2004/08/11 00:00 [revised]
PHST- 2004/09/24 00:00 [accepted]
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - S0168-6496(04)00278-8 [pii]
AID - 10.1016/j.femsec.2004.09.009 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2005 Feb 1;51(3):363-73. doi: 10.1016/j.femsec.2004.09.009. 
      Epub 2004 Oct 22.