PMID- 16329920
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20091119
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 52
IP  - 3
DP  - 2005 May 1
TI  - Food selection by bacterivorous protists: insight from the analysis of the food 
      vacuole content by means of fluorescence in situ hybridization.
PG  - 351-63
AB  - A modified fluorescence in situ hybridization (FISH) method was used to analyze 
      bacterial prey composition in protistan food vacuoles in both laboratory and 
      natural populations. Under laboratory conditions, we exposed two bacterial 
      strains (affiliated with beta- and gamma-Proteobacteria -- Aeromonas hydrophila 
      and Pseudomonas fluorescens, respectively) to grazing by three protists: the 
      flagellates Bodo saltans and Goniomonas sp., and the ciliate Cyclidium glaucoma. 
      Both flagellate species preferably ingested A. hydrophila over P. fluorescens, 
      while C. glaucoma showed no clear preferences. Differences were found in the 
      digestion of bacterial prey with B. saltans digesting significantly faster P. 
      fluorescens compared to two other protists. The field study was conducted in a 
      reservoir as part of a larger experiment. We monitored changes in the bacterial 
      prey composition available compared to the bacteria ingested in flagellate food 
      vacuoles. Bacteria detected by probe HGC69a (Actinobacteria) and R-BT065 were 
      negatively selected by flagellates. Bacteria detected by probe CF319a were 
      initially positively selected but along with a temporal shift in bacterial cell 
      size, this trend changed to negative selection during the experiment. Overall, 
      our analysis of protistan food vacuole content indicated marked effects of 
      flagellate prey selectivity on bacterioplankton community composition.
FAU - Jezbera, Jan
AU  - Jezbera J
AD  - Hydrobiological Institute of the Academy of Sciences of the Czech Republic, Na 
      Sadkach 7, CZ-37005, Ceske Budejovice, Czech Republic. jan.jezbera@email.cz
FAU - Hornak, Karel
AU  - Hornak K
FAU - Simek, Karel
AU  - Simek K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050119
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Aeromonas hydrophila/classification/genetics/*isolation & purification
MH  - Animals
MH  - Ciliophora/microbiology/*physiology/ultrastructure
MH  - DNA, Bacterial/analysis
MH  - DNA, Ribosomal/analysis
MH  - Eukaryota/microbiology/*physiology/ultrastructure
MH  - Feeding Behavior/*physiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Predatory Behavior/physiology
MH  - Pseudomonas fluorescens/classification/genetics/*isolation & purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rivers/microbiology/parasitology
MH  - Vacuoles/*microbiology
EDAT- 2005/12/07 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/12/07 09:00
PHST- 2004/10/16 00:00 [received]
PHST- 2004/11/26 00:00 [revised]
PHST- 2004/12/01 00:00 [accepted]
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - S0168-6496(04)00358-7 [pii]
AID - 10.1016/j.femsec.2004.12.001 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2005 May 1;52(3):351-63. doi: 10.1016/j.femsec.2004.12.001. 
      Epub 2005 Jan 19.