PMID- 16329938
OWN - NLM
STAT- MEDLINE
DCOM- 20060615
LR  - 20131121
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 53
IP  - 1
DP  - 2005 Jun 1
TI  - Anaerobic treatment of 2,4,6-trichlorophenol in an expanded granular sludge 
      bed-anaerobic filter (EGSB-AF) bioreactor at 15 degrees C.
PG  - 167-78
AB  - Expanded granular sludge bed-anaerobic filter (EGSB-AF) bioreactors were operated 
      at 15 degrees C for the treatment of 2,4,6-trichlorophenol (TCP)-containing 
      volatile fatty acid (VFA)-based wastewaters. The seed sludge used as inoculum for 
      the control (no TCP) and test reactor was unexposed to chlorophenols (CPs) prior 
      to the 425-day trial. TCP supplementation to the feed at 50 mg TCPl(-1) partially 
      inhibited the anaerobic degradation of the VFA feed measured as COD removal 
      efficiency. However, the withdrawal and subsequent application of stepwise 
      increments to the TCP loading resulted in steady COD removal. Terminal 
      restriction fragment length polymorphism analysis showed Methanosaeta-like 
      Archaea in the control reactor over the experimental period. Different 
      methanogenic populations were detected in the test reactor and responded to the 
      changes in feed composition. Bacterial community analyses indicated changes in 
      the community structure over time and suggested the presence of 
      Campylobacter-like, Acidimicrobium-like and Heliophilum-like organisms in the 
      samples. TCP mineralisation was by a reductive dechlorination pathway through 
      2,4-dichlorophenol (DCP) and 4-chlorophenol (4-CP) or 2-chlorophenol (2-CP). CP 
      degradation rates in sludge granules from the lower chamber of the hybrid EGSB-AF 
      reactor was in the order TCP > DCP > 4-CP > 2-CP. However, a biodegradability 
      order of lower CPs > TCP was observed in fixed-film biomass taken from the upper 
      reactor chamber, thus reflecting the role of this reactor section in the 
      metabolism of residual lower CPs from the lower sludge-bed stage of operation.
FAU - Collins, Gavin
AU  - Collins G
AD  - Microbial Ecology Laboratory, Department of Microbiology and Environmental Change 
      Institute (ECI), National University of Ireland, Galway, (NUI, Galway), Galway, 
      Ireland.
FAU - Foy, Clare
AU  - Foy C
FAU - McHugh, Sharon
AU  - McHugh S
FAU - O'Flaherty, Vincent
AU  - O'Flaherty V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041121
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Chlorophenols)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Water Pollutants, Chemical)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteria, Anaerobic/*metabolism
MH  - Biodegradation, Environmental
MH  - *Bioreactors
MH  - Chlorophenols/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Methane/analysis
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Species Specificity
MH  - Spectrophotometry, Ultraviolet
MH  - Temperature
MH  - Time Factors
MH  - Waste Disposal, Fluid/*methods
MH  - Water Pollutants, Chemical/*metabolism
EDAT- 2005/12/07 09:00
MHDA- 2006/06/16 09:00
CRDT- 2005/12/07 09:00
PHST- 2004/05/21 00:00 [received]
PHST- 2004/09/10 00:00 [revised]
PHST- 2004/10/22 00:00 [accepted]
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/06/16 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - S0168-6496(04)00305-8 [pii]
AID - 10.1016/j.femsec.2004.10.008 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2005 Jun 1;53(1):167-78. doi: 10.1016/j.femsec.2004.10.008. 
      Epub 2004 Nov 21.