PMID- 16329957
OWN - NLM
STAT- MEDLINE
DCOM- 20060601
LR  - 20240109
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 53
IP  - 3
DP  - 2005 Aug 1
TI  - Colonization and community dynamics of class Flavobacteria on diatom detritus in 
      experimental mesocosms based on Southern Ocean seawater.
PG  - 379-91
AB  - In order to better understand the ecology of microorganisms responsible for 
      secondary production in the Southern Ocean the activity of Flavobacteria 
      communities on diatom detritus in seawater mesocosms was investigated. Seawater 
      was collected from different parts of the Southern Ocean including the Polar 
      Front Zone (PFZ), ice-edge area of the Antarctic Zone (AZ), and a site in the AZ 
      ice pack. Detritus from the cosmopolitan marine diatom Nitzschia closterium 
      Ehrenberg was resuspended in mesocosms containing seawater filtered to remove 
      particulate organic matter, including particle-associated bacteria and most 
      eukaryotes, but retaining native planktonic bacterial assemblages. Mesocosms were 
      incubated at 2 degrees C and samples analysed for changes in community 
      composition using denaturing gradient gel electrophoresis (DGGE), real-time PCR 
      and fluorescent in-situ hybridization (FISH). DGGE banding patterns and FISH 
      images demonstrated rapid bacterial colonization of the detritus, dominated by 
      members of class gamma-Proteobacteria, alpha-Proteobacteria and Flavobacteria. 
      Real-time PCR data indicated members of class Flavobacteria were involved in 
      initial colonization of detrital aggregate, however relative abundance stayed at 
      similar levels found for the original native particle-associated populations. 16S 
      rRNA gene DGGE banding patterns and sequence analysis demonstrated significant 
      variation in Flavobacteria community structure occurred in the first 20 days of 
      the experiment before community stabilization occurred. The community structures 
      between the three mesocosms also markedly differed and major colonizers were 
      primarily derived from detectable members of the initial particle-associated 
      Flavobacteria community, however the abundant uncultured Flavobacteria agg58 
      clone-related and DE cluster 2 clades, previously identified in Southern Ocean 
      seawater were not observed to colonize the detritus.
FAU - Abell, G C J
AU  - Abell GC
AD  - School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, 
      Tasmania 7001, Australia.
FAU - Bowman, John P
AU  - Bowman JP
LA  - eng
SI  - GENBANK/AY285940
SI  - GENBANK/AY285941
SI  - GENBANK/AY285942
SI  - GENBANK/AY285943
SI  - GENBANK/AY285944
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SI  - GENBANK/AY298788
SI  - GENBANK/AY319330
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SI  - GENBANK/AY533199
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SI  - GENBANK/AY661596
SI  - GENBANK/AY661597
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SI  - GENBANK/AY661599
SI  - GENBANK/AY661600
SI  - GENBANK/AY661601
SI  - GENBANK/AY661602
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SI  - GENBANK/AY661609
SI  - GENBANK/AY661610
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - DNA, Bacterial/analysis/genetics
MH  - *Diatoms
MH  - Electrophoresis/methods
MH  - Flavobacterium/genetics/*isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oceans and Seas
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Proteobacteria/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
MH  - Silicon Dioxide/analysis
MH  - Water Microbiology
EDAT- 2005/12/07 09:00
MHDA- 2006/06/02 09:00
CRDT- 2005/12/07 09:00
PHST- 2004/10/24 00:00 [received]
PHST- 2005/01/07 00:00 [revised]
PHST- 2005/01/21 00:00 [accepted]
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/06/02 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - S0168-6496(05)00025-5 [pii]
AID - 10.1016/j.femsec.2005.01.008 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2005 Aug 1;53(3):379-91. doi: 10.1016/j.femsec.2005.01.008.