PMID- 16329964
OWN - NLM
STAT- MEDLINE
DCOM- 20060601
LR  - 20061115
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 53
IP  - 3
DP  - 2005 Aug 1
TI  - Application of cation-exchange membranes for characterisation and imaging 
      ammonia-oxidising bacteria in soils.
PG  - 463-72
AB  - A new approach, in which ammonia-oxidizing bacteria (AOB) are entrapped from soil 
      onto cation-exchange membranes, was applied to identify terrestrial AOB by 
      fluorescence in situ hybridization (FISH). An experimental hot spot of ammonia 
      oxidation was developed by establishing a gradient of ammonium substrate (200 to 
      <20 mg NH4+-N l(-1)) diffused through the cation-exchange membranes incubated in 
      soil for 6 months. By this approach we were able to characterise and image 
      indigenous AOB populations growing in heavily oil-polluted soil using FISH and 
      sequence analysis of PCR-amplified 16S rRNA genes, respectively. The FISH results 
      revealed that Nitrosospira-like AOB were dominant on the ammonium-enriched 
      membranes incubated in the soil. Fourteen unique Nitrosospira-like 16S rRNA gene 
      sequences belonging to clusters 2 and 3 were recovered from the soil-incubated 
      membranes and from the soil, suggesting the importance of Nitrosospira-like AOB 
      in the oil-polluted landfarming soil.
FAU - Kurola, Jukka
AU  - Kurola J
AD  - Department of Biological and Environmental Sciences, Division of General 
      Microbiology, FIN-00014 University of Helsinki, Finland. Jukka.Kurola@Helsinki.fi
FAU - Wittmann, Christoph
AU  - Wittmann C
FAU - Salkinoja-Salonen, Mirja
AU  - Salkinoja-Salonen M
FAU - Aarnio, Tuula
AU  - Aarnio T
FAU - Romantschuk, Martin
AU  - Romantschuk M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Cation Exchange Resins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Industrial Waste)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Petroleum)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Betaproteobacteria/classification/genetics/*isolation & purification/metabolism
MH  - Cation Exchange Resins
MH  - Colony Count, Microbial
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Finland
MH  - In Situ Hybridization, Fluorescence
MH  - Industrial Waste
MH  - Membranes, Artificial
MH  - Oxidation-Reduction
MH  - *Petroleum
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Soil Microbiology
MH  - Soil Pollutants/*metabolism
EDAT- 2005/12/07 09:00
MHDA- 2006/06/02 09:00
CRDT- 2005/12/07 09:00
PHST- 2004/07/06 00:00 [received]
PHST- 2004/11/05 00:00 [revised]
PHST- 2005/02/03 00:00 [accepted]
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/06/02 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - S0168-6496(05)00037-1 [pii]
AID - 10.1016/j.femsec.2005.02.001 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2005 Aug 1;53(3):463-72. doi: 10.1016/j.femsec.2005.02.001.