PMID- 16330051
OWN - NLM
STAT- MEDLINE
DCOM- 20060622
LR  - 20101118
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 132
IP  - 1
DP  - 2006 May
TI  - Modulation of monocyte-derived dendritic cell differentiation is associated with 
      ischemic acute renal failure.
PG  - 104-11
AB  - BACKGROUND: Dendritic cells (DCs) play a central role in both stimulating and 
      suppressing immune responses and are impacted by surgical injury, exercise, and 
      other physiological stressors. This study aims to determine whether renal 
      ischemia/reperfusion (I/R) injury alters the differentiation, maturation, and 
      activation of DCs from peripheral blood monocytes (PBMo). MATERIALS AND METHODS: 
      Sprague-Dawley (SD) rats were subjected to I/R injury or sham-operated. 
      Creatinine clearance (CCr) was monitored daily during the 14 days of reperfusion 
      that followed the ischemic insult. At 2 and 14 days of reperfusion, the following 
      properties of PBMo derived-DCs were assessed: the amount of generated DCs, 
      surface markers [CD11c, CD80, CD86, and MHC-II (IA)], and functional status 
      including magnitude of mixed lymphocyte reaction (MLR), production of IL-12 p70 
      by DCs, and production of IFN-gamma and IL-4 by DC-stimulated T cells. RESULTS: 
      CCr was greatly reduced in the injured rats 0 to 4 days after ischemia. Two days 
      after I/R injury to kidney, the numbers of DCs differentiated from PBMo, IL-12 
      production by DCs, expression of MHC-II (IA), and IFN-gamma production by 
      DC-stimulated T cells were significantly increased in the I/R injured group 
      (compared to the sham-operated group). After 14 days of reperfusion, there was no 
      between-group differences in the numbers of DCs derived from PBMo, MLR, 
      expression of CD80, CD86, and MHC-II (IA), and production of IL-12, IFN-gamma, 
      and IL-4. CONCLUSIONS: The increases seen at 2 days of reperfusion may reflect a 
      preparatory step in the renal I/R injury pathway. The relationship between 
      up-regulation of DC differentiation and ischemic acute renal failure (ARF) 
      remains to be elucidated.
FAU - Wu, Chih-Jen
AU  - Wu CJ
AD  - Graduate Institute of Medical Science, Taipei Medical University, Taipei, Taiwan.
FAU - Sheu, Joen-Rong
AU  - Sheu JR
FAU - Chen, Han-Hsiang
AU  - Chen HH
FAU - Liao, Hui-Fen
AU  - Liao HF
FAU - Yang, Yuh-Cheng
AU  - Yang YC
FAU - Yang, Stone
AU  - Yang S
FAU - Chen, Yu-Jen
AU  - Chen YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051205
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Acute Kidney Injury/*immunology/*physiopathology
MH  - Animals
MH  - Antigens, Differentiation/analysis
MH  - Cell Differentiation
MH  - Dendritic Cells/*immunology/pathology
MH  - Disease Models, Animal
MH  - Histocompatibility Antigens Class II/analysis
MH  - Interferon-gamma/blood
MH  - Interleukin-4/blood
MH  - Ischemia/*immunology/*physiopathology
MH  - Monocytes/*immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Renal Circulation
MH  - Reperfusion Injury/immunology
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2005/12/07 09:00
MHDA- 2006/06/23 09:00
CRDT- 2005/12/07 09:00
PHST- 2005/06/01 00:00 [received]
PHST- 2005/09/07 00:00 [revised]
PHST- 2005/09/29 00:00 [accepted]
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/06/23 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - S0022-4804(05)00529-9 [pii]
AID - 10.1016/j.jss.2005.09.029 [doi]
PST - ppublish
SO  - J Surg Res. 2006 May;132(1):104-11. doi: 10.1016/j.jss.2005.09.029. Epub 2005 Dec 
      5.