PMID- 16331327
OWN - NLM
STAT- MEDLINE
DCOM- 20060223
LR  - 20191210
IS  - 1672-9145 (Print)
IS  - 1672-9145 (Linking)
VI  - 37
IP  - 12
DP  - 2005 Dec
TI  - Cloning and characterization of a new isoform of mouse interleukin-18.
PG  - 826-34
AB  - Interleukin-18 (IL-18) is a novel proinflammatory cytokine with potent interferon 
      (IFN)-lambda inducing activity that plays an important biological role in the 
      enhancement of the activity of natural killer cells and cytotoxic T lymphocytes. 
      In this study, we have identified a novel short form of IL-18 in mouse, named 
      IL-18s. IL-18s might be an alternative splicing variant of IL-18 and its cDNA 
      contains a 57 bp in-frame deletion. Like IL-18, IL-18s is also widely expressed 
      in mouse tissues. It was suggested that IL-18s might have a caspase-1-dependent 
      mechanism for maturation and secretion similar to that of IL-18: when transfected 
      in COS-7 cells, pro-IL-18s (22 kDa) could be detected, and the mature IL-18s (16 
      kDa) could also be detected when combined with caspase-1. We observed that 
      recombinant mouse IL-18s did not show any IL-18-like function, and IL-18s could 
      enhance the ability of IL-18 to increase IFN-lambda production by approximately 
      40% in mouse splenocytes. This effect was observed primarily at relative low 
      concentrations of IL-18, suggesting that IL-18s might regulate the activity of 
      IL-18 in the physiological conditions.
FAU - Yang, Yong-Jie
AU  - Yang YJ
AD  - Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological 
      Sciences, Chinese Academy of Sciences; Graduate School of the Chinese Academy of 
      Sciences, Shanghai 200031 China.
FAU - Wang, Zhao-Yuan
AU  - Wang ZY
FAU - Chen, Song-Hua
AU  - Chen SH
FAU - Ge, Xi-Rui
AU  - Ge XR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Interleukin-18)
RN  - 0 (Protein Isoforms)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/*chemistry/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Protein Isoforms/chemistry/*metabolism
EDAT- 2005/12/07 09:00
MHDA- 2006/02/24 09:00
CRDT- 2005/12/07 09:00
PHST- 2005/12/07 09:00 [pubmed]
PHST- 2006/02/24 09:00 [medline]
PHST- 2005/12/07 09:00 [entrez]
AID - 10.1111/j.1745-7270.2005.00116.x [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2005 Dec;37(12):826-34. doi: 
      10.1111/j.1745-7270.2005.00116.x.