PMID- 16332746
OWN - NLM
STAT- MEDLINE
DCOM- 20060207
LR  - 20181203
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 12
DP  - 2005 Dec
TI  - Activity and phylogenetic diversity of bacterial cells with high and low nucleic 
      acid content and electron transport system activity in an upwelling ecosystem.
PG  - 7737-49
AB  - We evaluated whether bacteria with higher cell-specific nucleic acid content 
      (HNA) or an active electron transport system, i.e., positive for reduction of 
      5-cyano-2,3-ditolyl tetrazolium chloride (CTC), were responsible for the bulk of 
      bacterioplankton metabolic activity. We also examined whether the phylogenetic 
      diversity of HNA and CTC-positive cells differed from the diversity of Bacteria 
      with low nucleic acid content (LNA). Bacterial assemblages were sampled both in 
      eutrophic shelf waters and in mesotrophic offshore waters in the Oregon coastal 
      upwelling region. Cytometrically sorted HNA, LNA, and CTC-positive cells were 
      assayed for their cell-specific [3H]leucine incorporation rates. Phylogenetic 
      diversity in sorted non-radioactively labeled samples was assayed using 
      denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA genes. 
      Cell-specific rates of leucine incorporation of HNA and CTC-positive cells were 
      on average only slightly greater than the cell-specific rates of LNA cells. HNA 
      cells accounted for most bacterioplankton substrate incorporation due to high 
      abundances, while the low abundances of CTC-positive cells resulted in only a 
      small contribution by these cells to total bacterial activity. The proportion of 
      the total bacterial leucine incorporation attributable to LNA cells was higher in 
      offshore regions than in shelf waters. Sequence data obtained from DGGE bands 
      showed broadly similar phylogenetic diversity across HNA, LNA, and CTC-positive 
      cells, with between-sample and between-region variability in the distribution of 
      phylotypes. Our results suggest that LNA bacteria are not substantially different 
      from HNA bacteria in either cell-specific rates of substrate incorporation or 
      phylogenetic composition and that they can be significant contributors to 
      bacterial metabolism in the sea.
FAU - Longnecker, K
AU  - Longnecker K
AD  - OSU/COAS, 104 COAS Admin Bldg., Corvallis, OR 97331-5503, USA. 
      klongnecker@coas.oregonstate.edu
FAU - Sherr, B F
AU  - Sherr BF
FAU - Sherr, E B
AU  - Sherr EB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Nitrates)
RN  - 0 (Nucleic Acids)
RN  - 1406-65-1 (Chlorophyll)
RN  - GMW67QNF9C (Leucine)
RN  - YF5Q9EJC8Y (Chlorophyll A)
SB  - IM
MH  - Bacteria/*classification/*metabolism
MH  - Chlorophyll/metabolism
MH  - Chlorophyll A
MH  - *Ecosystem
MH  - Electron Transport
MH  - Leucine/*metabolism
MH  - Nitrates/metabolism
MH  - Nucleic Acids/analysis/*metabolism
MH  - Phylogeny
MH  - Seawater/microbiology
MH  - Temperature
PMC - PMC1317353
EDAT- 2005/12/08 09:00
MHDA- 2006/02/08 09:00
PMCR- 2005/12/01
CRDT- 2005/12/08 09:00
PHST- 2005/12/08 09:00 [pubmed]
PHST- 2006/02/08 09:00 [medline]
PHST- 2005/12/08 09:00 [entrez]
PHST- 2005/12/01 00:00 [pmc-release]
AID - 71/12/7737 [pii]
AID - 0839-05 [pii]
AID - 10.1128/AEM.71.12.7737-7749.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Dec;71(12):7737-49. doi: 
      10.1128/AEM.71.12.7737-7749.2005.