PMID- 16332875 OWN - NLM STAT- MEDLINE DCOM- 20060207 LR - 20220330 IS - 0099-2240 (Print) IS - 1098-5336 (Electronic) IS - 0099-2240 (Linking) VI - 71 IP - 12 DP - 2005 Dec TI - Modification of surface properties of biomaterials influences the ability of Candida albicans to form biofilms. PG - 8795-801 AB - Candida albicans biofilms form on indwelling medical devices (e.g., denture acrylic or intravenous catheters) and are associated with both oral and invasive candidiasis. Here, we determined whether surface modifications of polyetherurethane (Elasthane 80A [E80A]), polycarbonateurethane, and poly(ethyleneterephthalate) (PET) can influence fungal biofilm formation. Polyurethanes were modified by adding 6% polyethylene oxide (6PEO), 6% fluorocarbon, or silicone, while the PET surface was modified to generate hydrophilic, hydrophobic, cationic, or anionic surfaces. Formation of biofilm was quantified by determining metabolic activity and total biomass (dry weight), while its architecture was analyzed by confocal scanning laser microscopy (CSLM). The metabolic activity of biofilm formed by C. albicans on 6PEO-E80A was significantly reduced (by 78%) compared to that of biofilm formed on the nonmodified E80A (optical densities of 0.054 +/- 0.020 and 0.24 +/- 0.10, respectively; P = 0.037). The total biomass of Candida biofilm formed on 6PEO-E80A was 74% lower than that on the nonmodified E80A surface (0.46 +/- 0.15 versus 1.76 +/- 0.32 mg, respectively; P = 0.003). Fungal cells were easily detached from the 6PEO-E80A surface, and we were unable to detect C. albicans biofilm on this surface by CSLM. All other surface modifications allowed formation of C. albicans biofilm, with some differences in thearchitecture. Correlation between contact angle and biofilm formation was observed for polyetherurethane substrates (r = 0.88) but not for PET biomaterials (r = -0.40). This study illustrates that surface modification is a viable approach for identifying surfaces that have antibiofilm characteristics. Investigations into the clinical utility of the identified surfaces are warranted. FAU - Chandra, Jyotsna AU - Chandra J AD - Center for Medical Mycology, Department of Dermatology, University Hospitals of Cleveland and Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106-5028, USA. FAU - Patel, Jasmine D AU - Patel JD FAU - Li, Jian AU - Li J FAU - Zhou, Guangyin AU - Zhou G FAU - Mukherjee, Pranab K AU - Mukherjee PK FAU - McCormick, Thomas S AU - McCormick TS FAU - Anderson, James M AU - Anderson JM FAU - Ghannoum, Mahmoud A AU - Ghannoum MA LA - eng GR - P30-AR-39750/AR/NIAMS NIH HHS/United States GR - P30 AR039750/AR/NIAMS NIH HHS/United States GR - R01 DE013932/DE/NIDCR NIH HHS/United States GR - P30 CA043703/CA/NCI NIH HHS/United States GR - P30CA43703-12/CA/NCI NIH HHS/United States GR - 1R01 DE13932-01A1/DE/NIDCR NIH HHS/United States GR - R01 EB000279/EB/NIBIB NIH HHS/United States GR - EB 00279/EB/NIBIB NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 RN - 0 (Biocompatible Materials) RN - 0 (Fluorocarbons) RN - 0 (Polycarboxylate Cement) RN - 0 (Silicones) RN - 25766-59-0 (polycarbonate) RN - 3IN71E75Z5 (Urethane) RN - 3WJQ0SDW1A (Polyethylene Glycols) SB - IM MH - *Biocompatible Materials MH - *Biofilms MH - Candida albicans/cytology/*growth & development MH - Fluorocarbons MH - Polycarboxylate Cement MH - Polyethylene Glycols MH - Silicones MH - Surface Properties MH - Urethane PMC - PMC1317330 EDAT- 2005/12/08 09:00 MHDA- 2006/02/08 09:00 PMCR- 2005/12/01 CRDT- 2005/12/08 09:00 PHST- 2005/12/08 09:00 [pubmed] PHST- 2006/02/08 09:00 [medline] PHST- 2005/12/08 09:00 [entrez] PHST- 2005/12/01 00:00 [pmc-release] AID - 71/12/8795 [pii] AID - 0565-05 [pii] AID - 10.1128/AEM.71.12.8795-8801.2005 [doi] PST - ppublish SO - Appl Environ Microbiol. 2005 Dec;71(12):8795-801. doi: 10.1128/AEM.71.12.8795-8801.2005.