PMID- 16333823
OWN - NLM
STAT- MEDLINE
DCOM- 20060307
LR  - 20200930
IS  - 1552-4825 (Print)
IS  - 1552-4825 (Linking)
VI  - 140
IP  - 1
DP  - 2006 Jan 1
TI  - Investigation of confined placental mosaicism (CPM) at multiple sites in 
      post-delivery placentas derived through intracytoplasmic sperm injection (ICSI).
PG  - 24-30
AB  - Although earlier studies on pregnancies derived through intracytoplasmic sperm 
      injection (ICSI) reported increased non-mosaic aneuploidy among ICSI children, 
      undetected mosaicism, such as confined placental mosaicism (CPM) has not been 
      evaluated. We investigated the incidence of CPM in post-delivery placentas 
      derived from ICSI, evaluated whether CPM was increased and whether it was a 
      contributing factor to negative pregnancy outcome. [Fifty-one post-delivery 
      placentas were collected from patients who underwent ICSI with a normal or 
      negative pregnancy outcome]. Trophoblast and chorionic stroma from three sites 
      were analyzed by comparative genomic hybridization (CGH) and flow cytometry. 
      Detected abnormalities were confirmed by fluorescence in situ hybridization 
      (FISH). The incidence of CPM in the ICSI population was compared to the general 
      population from published data. We detected three cases of CPM in our study. One 
      abnormality was found by CGH analysis; partial trisomy 7q and a partial monosomy 
      Xp limited to the trophoblast at two sites. The abnormality was associated with a 
      child affected by spina bifida. Two cases of mosaic tetraploidy were observed by 
      flow cytometry in pregnancies with a normal outcome. All three abnormalities were 
      confirmed by FISH analysis. The incidence of CPM in the ICSI study population was 
      5.88% (3/51), which was not statistically different from published reports in the 
      general population (5.88% (42/714), Chi square, P > 0.05). The post-ICSI 
      population was not at risk for CPM in this study.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Minor, Agata
AU  - Minor A
AD  - Department of Obstetrics and Gynecology, University of British Columbia, 855 West 
      12th Avenue, Vancouver, British Columbia V5Z 1M9, Canada.
FAU - Harmer, Karynn
AU  - Harmer K
FAU - Peters, Nicole
AU  - Peters N
FAU - Yuen, Basil Ho
AU  - Yuen BH
FAU - Ma, Sai
AU  - Ma S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Med Genet A
JT  - American journal of medical genetics. Part A
JID - 101235741
SB  - IM
MH  - Adult
MH  - Chi-Square Distribution
MH  - Female
MH  - Flow Cytometry
MH  - Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Mosaicism
MH  - Nucleic Acid Hybridization/methods
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - *Sperm Injections, Intracytoplasmic
EDAT- 2005/12/08 09:00
MHDA- 2006/03/08 09:00
CRDT- 2005/12/08 09:00
PHST- 2005/12/08 09:00 [pubmed]
PHST- 2006/03/08 09:00 [medline]
PHST- 2005/12/08 09:00 [entrez]
AID - 10.1002/ajmg.a.31016 [doi]
PST - ppublish
SO  - Am J Med Genet A. 2006 Jan 1;140(1):24-30. doi: 10.1002/ajmg.a.31016.