PMID- 16337347
OWN - NLM
STAT- MEDLINE
DCOM- 20060718
LR  - 20061115
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 113
IP  - 3-4
DP  - 2006 Mar 31
TI  - Temporary inhibition of neuronal apoptosis in Aujeszky's disease virus-infected 
      swine.
PG  - 237-42
AB  - Previous studies have shown that during acute infection of the porcine trigeminal 
      ganglia (TG), Aujeszky's disease virus (ADV)-infected neurons are protected from 
      apoptosis induced by the virus itself and by cells of the immune system. However, 
      TG neurons productively infected by ADV finally die and are phagocytosed by 
      adjacent cells, a fact that leads us to speculate that the inhibition of neuronal 
      apoptosis by ADV may be temporary rather than absolute. To address this issue we 
      used TG and brain stem from pigs during acute infection by ADV. Infected cells 
      were detected by immunohistochemical staining of viral antigens, whereas 
      apoptotic cells were identified with an anti-active caspase-3 antibody, the TUNEL 
      assay and by transmission electron microscopy. The results obtained in this study 
      support the contention that the inhibition of neuronal apoptosis by ADV is 
      temporary, since activation of caspase-3 could be detected in infected neurons at 
      late stages in infection and because foci of advanced neuronophagia contained 
      neurons exhibiting typical ultrastructural features of apoptosis.
FAU - Marcaccini, A
AU  - Marcaccini A
AD  - Catedra de Histologia II y Embriologia Especial, Facultad de Ciencias 
      Veterinarias, Universidad Nacional de Rosario, Casilda, Argentina.
FAU - Aleman, N
AU  - Aleman N
FAU - Quiroga, M I
AU  - Quiroga MI
FAU - Lopez, M
AU  - Lopez M
FAU - Guerrero, F
AU  - Guerrero F
FAU - Nieto, J M
AU  - Nieto JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051206
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - *Apoptosis
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Herpesvirus 1, Suid/*pathogenicity
MH  - Immunohistochemistry/veterinary
MH  - In Situ Nick-End Labeling/veterinary
MH  - Neurons/cytology/enzymology/*virology
MH  - Pseudorabies/pathology/*virology
MH  - Swine
MH  - Swine Diseases/pathology/*virology
MH  - Trigeminal Ganglion/physiology/ultrastructure/*virology
MH  - Virus Latency
EDAT- 2005/12/13 09:00
MHDA- 2006/07/19 09:00
CRDT- 2005/12/13 09:00
PHST- 2005/12/13 09:00 [pubmed]
PHST- 2006/07/19 09:00 [medline]
PHST- 2005/12/13 09:00 [entrez]
AID - S0378-1135(05)00380-9 [pii]
AID - 10.1016/j.vetmic.2005.11.039 [doi]
PST - ppublish
SO  - Vet Microbiol. 2006 Mar 31;113(3-4):237-42. doi: 10.1016/j.vetmic.2005.11.039. 
      Epub 2005 Dec 6.