PMID- 16337823
OWN - NLM
STAT- MEDLINE
DCOM- 20060811
LR  - 20220311
IS  - 1344-6223 (Print)
IS  - 1344-6223 (Linking)
VI  - 8
IP  - 2
DP  - 2006 Mar
TI  - Real-time RT-PCR quantitative assays and postmortem degradation profiles of 
      erythropoietin, vascular endothelial growth factor and hypoxia-inducible factor 1 
      alpha mRNA transcripts in forensic autopsy materials.
PG  - 132-6
AB  - Recent advances in molecular biology have suggested the potential usefulness of 
      mRNA analyses in postmortem investigations of fatal mechanisms. The aim of the 
      present study was to establish quantitative assays of oxygen-regulated factors 
      including erythropoietin (EPO), vascular endothelial growth factor (VEGF) and 
      hypoxia-inducible factor 1 alpha (HIF1A) mRNAs, and to investigate the postmortem 
      stability of these mRNA transcripts in forensic autopsy materials. Relative 
      quantification of EPO, VEGF and HIF1A mRNAs, based on the TaqMan reverse 
      transcription-polymerase chain reaction (RT-PCR), was performed on autopsy tissue 
      specimens from the heart (n = 10), brain (n = 10), kidney (n = 16) and lung (n = 
      8) after preservation at room temperature for various storage times. VEGF and 
      HIF1A mRNA gradually degraded in patterns similar to glyceraldehydes-3-phosphate 
      dehydrogenase (GAPDH) mRNA used as an endogenous reference. Accordingly, the 
      relative quantification of VEGF/GAPDH and HIF1A/GAPDH changed little up to 48h 
      postmortem in tissue samples from the brain, kidney and lung except for a mild 
      deviation of HIF1A in the myocardium. However, the status was different for EPO 
      mRNA, with extraordinary stability for postmortem degradation and a marked 
      postmortem time-dependent increase in the EPO/GAPDH ratio for all tissue samples. 
      The present study suggested the potential for applying quantitative analyses of 
      mRNA transcripts to autopsy materials and indicated the significance of 
      investigating degradation profiles prior to carrying out relative quantification 
      of target mRNAs in autopsy materials.
FAU - Zhao, Dong
AU  - Zhao D
AD  - Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 
      1-4-3, Abeno, Osaka 545-8585, Japan. legalmed@med.osaka-cu.ac.jp
FAU - Zhu, Bao-Li
AU  - Zhu BL
FAU - Ishikawa, Takaki
AU  - Ishikawa T
FAU - Quan, Li
AU  - Quan L
FAU - Li, Dong-Ri
AU  - Li DR
FAU - Maeda, Hitoshi
AU  - Maeda H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051207
PL  - Ireland
TA  - Leg Med (Tokyo)
JT  - Legal medicine (Tokyo, Japan)
JID - 100889186
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - Brain Chemistry
MH  - Erythropoietin/*chemistry/genetics
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*chemistry/genetics
MH  - Kidney/chemistry
MH  - Lung/chemistry
MH  - Myocardium/chemistry
MH  - *Postmortem Changes
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Specimen Handling
MH  - Vascular Endothelial Growth Factor A/*chemistry/genetics
EDAT- 2005/12/13 09:00
MHDA- 2006/08/12 09:00
CRDT- 2005/12/13 09:00
PHST- 2005/07/13 00:00 [received]
PHST- 2005/09/12 00:00 [revised]
PHST- 2005/09/28 00:00 [accepted]
PHST- 2005/12/13 09:00 [pubmed]
PHST- 2006/08/12 09:00 [medline]
PHST- 2005/12/13 09:00 [entrez]
AID - S1344-6223(05)00122-7 [pii]
AID - 10.1016/j.legalmed.2005.09.001 [doi]
PST - ppublish
SO  - Leg Med (Tokyo). 2006 Mar;8(2):132-6. doi: 10.1016/j.legalmed.2005.09.001. Epub 
      2005 Dec 7.