PMID- 16339168
OWN - NLM
STAT- MEDLINE
DCOM- 20060509
LR  - 20220419
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 21
IP  - 3
DP  - 2006 Mar
TI  - Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of 
      chromosomal translocation carrier patients.
PG  - 685-93
AB  - BACKGROUND: To try to explain the infertility of chromosomal translocation 
      carrier patients, we compared the expression of two markers of apoptosis in the 
      sperm of patients and of fertile donors, and we studied the meiotic segregation 
      in the ejaculated sperm of these translocation carriers. METHODS: Twenty semen 
      samples of translocation carriers, [reciprocal (n=14) and Robertsonian 
      translocations (n=6)], were compared with the semen samples of donors (n=20). 
      Different tests were applied: annexin V binding assay; terminal deoxynucleotidyl 
      transferase-mediated dUTP-biotin nick end labelling (TUNEL); and fluorescence in 
      situ hybridization (FISH). RESULTS: The annexin V binding assay in sperm of 
      patients with chromosomal translocation (n=17) showed a significantly increased 
      proportion of sperm with externalized phosphatidylserine (PS) than in the control 
      group (n=20, P<or=0.05). The rates of DNA fragmentation investigated by TUNEL 
      reaction were higher in samples of translocation carriers (n=14) than in donors 
      (n=20, P<0.0001). The measures by FISH technique showed that the proportions of 
      balanced or normal gametes were predominant in the reciprocal translocation group 
      (alternate: n=7; from 33.0 to 58.8%; adjacent I: n=7; from 4.6 to 43.8%) and in 
      the Robertsonian translocation group (normal: n=5; from 76.0 to 88.5%). 
      CONCLUSIONS: Our data show a predominant proportion of balanced gametes in sperm 
      of chromosomal translocation carrier patients. Moreover, PS externalization and 
      DNA fragmentation rates are significantly higher in ejaculated sperm of these 
      patients than in donor sperm. These tests could be used to predict the outcome of 
      ICSI for these patients.
FAU - Brugnon, F
AU  - Brugnon F
AD  - Biologie de la Reproduction, CECOS, Departement de gynecologie obstetrique et 
      reproduction humaine and Research Centre Reproductive and Genetics, Vrije 
      Universiteit Brussel, Laarbeeklaan 101, B-1090, Belgium. 
      fbrugnon@chu-clermontferrand.fr
FAU - Van Assche, E
AU  - Van Assche E
FAU - Verheyen, G
AU  - Verheyen G
FAU - Sion, B
AU  - Sion B
FAU - Boucher, D
AU  - Boucher D
FAU - Pouly, J L
AU  - Pouly JL
FAU - Janny, L
AU  - Janny L
FAU - Devroey, P
AU  - Devroey P
FAU - Liebaers, I
AU  - Liebaers I
FAU - Van Steirteghem, A
AU  - Van Steirteghem A
LA  - eng
PT  - Journal Article
DEP - 20051208
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Annexin A5)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Annexin A5/analysis
MH  - Apoptosis/*physiology
MH  - Biomarkers/analysis
MH  - Chromosome Mapping
MH  - Ejaculation
MH  - *Genetic Carrier Screening
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - In Situ Nick-End Labeling
MH  - Karyotyping
MH  - Male
MH  - Meiosis
MH  - Spermatozoa/cytology/*physiology
MH  - Translocation, Genetic/*genetics
EDAT- 2005/12/13 09:00
MHDA- 2006/05/10 09:00
CRDT- 2005/12/13 09:00
PHST- 2005/12/13 09:00 [pubmed]
PHST- 2006/05/10 09:00 [medline]
PHST- 2005/12/13 09:00 [entrez]
AID - dei401 [pii]
AID - 10.1093/humrep/dei401 [doi]
PST - ppublish
SO  - Hum Reprod. 2006 Mar;21(3):685-93. doi: 10.1093/humrep/dei401. Epub 2005 Dec 8.