PMID- 16341640
OWN - NLM
STAT- MEDLINE
DCOM- 20061017
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 50
IP  - 4
DP  - 2005 Nov
TI  - Effects of viruses and predators on prokaryotic community composition.
PG  - 557-69
AB  - Dialysis bags were used to examine the impact of predation and viral lysis on 
      prokaryotic community composition (PCC) over a 5-day experiment in the 
      oligomesotrophic Lake Pavin (France). The impact of the different predator 
      communities (protists and metazoans) of prokaryotes was estimated by water 
      fractionation (<5 microm: treatment filtered on 5 microm, without ciliates and 
      metazoans; UNF: unfiltered treatment with all planktonic communities). 
      Enrichments of natural viruses (<1.2 microm: with a natural virus concentration; 
      <1.2 mum V and VV: with enrichment leading to a double or triple concentration of 
      viruses, respectively) were used to indirectly assess the control of 
      virioplankton. Viral activity was estimated from the frequency of visibly 
      infected cells (FVIC). PCC was determined by fluorescence in situ hybridization 
      (FISH) and terminal restriction fragment length polymorphism (T-RFLP). In this 
      study, PCC was affected by the eukaryote communities (especially flagellates), 
      and viruses to a lesser extent. Cyanobacteria declined significantly during the 
      experiment and were highly correlated with the FVIC. In addition, the 503-bp 
      terminal restriction fragment (T-RF) disappeared in treatments with virus 
      enrichments, suggesting possible viral-associated mortality processes, whereas 
      the 506-bp T-RF was not affected in these treatments. On one hand, these results 
      suggest a control of the PCC: first, by viral lysis of some dominant phylotypes 
      and second, by interspecific competition between resistant strains for the uptake 
      of substrates released by this lysis. The increase of Archaea may suggest that 
      these cells benefit such resources. On the other hand, the disappearance and the 
      stable proportion of some dominant phylotypes suggested a selection pressure due 
      to the predatory activity on prokaryotes. In conclusion, prokaryotic abundance 
      appears to be mainly controlled by flagellate protists, which also affected PCC, 
      whereas viruses seemed to be essentially responsible for profound changes in PCC 
      via direct and indirect actions.
FAU - Jardillier, Ludwig
AU  - Jardillier L
AD  - Laboratoire de Biologie des Protistes UMR CNRS 6023, Universite Blaise Pascal, 
      Clermont-Ferrand II, 63177, Aubiere, France.
FAU - Bettarel, Yvan
AU  - Bettarel Y
FAU - Richardot, Mathilde
AU  - Richardot M
FAU - Bardot, Corinne
AU  - Bardot C
FAU - Amblard, Christian
AU  - Amblard C
FAU - Sime-Ngando, Telesphore
AU  - Sime-Ngando T
FAU - Debroas, Didier
AU  - Debroas D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051215
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics/*virology
MH  - Biodiversity
MH  - Ecosystem
MH  - Lysogeny
MH  - Mortality
MH  - Polymorphism, Restriction Fragment Length
MH  - Population Dynamics
MH  - Predatory Behavior
MH  - Prokaryotic Cells/classification/*virology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Time Factors
MH  - Viruses/genetics/*pathogenicity
MH  - *Water Microbiology
EDAT- 2005/12/13 09:00
MHDA- 2006/10/18 09:00
CRDT- 2005/12/13 09:00
PHST- 2005/02/16 00:00 [received]
PHST- 2005/06/13 00:00 [accepted]
PHST- 2005/12/13 09:00 [pubmed]
PHST- 2006/10/18 09:00 [medline]
PHST- 2005/12/13 09:00 [entrez]
AID - 10.1007/s00248-005-5030-y [doi]
PST - ppublish
SO  - Microb Ecol. 2005 Nov;50(4):557-69. doi: 10.1007/s00248-005-5030-y. Epub 2005 Dec 
      15.