PMID- 16342474
OWN - NLM
STAT- MEDLINE
DCOM- 20060201
LR  - 20170214
IS  - 0748-2337 (Print)
IS  - 0748-2337 (Linking)
VI  - 21
IP  - 9
DP  - 2005 Oct
TI  - Effect of 2,4-dichlorophenoxyacetic acid on the activities of some metabolic 
      enzymes for generating pyridine nucleotide pool of cells from mouse liver.
PG  - 231-7
AB  - 2,4-Dichlorophenoxyacetic acid (2,4-D), which is a plant auxin analogue, is 
      lethal to broad leaved weeds within days at high dosages and is considered as 
      having low toxicity to mammals. Some studies have reported that exposure to this 
      compound may cause damage to organs such as liver. The aim of this study was to 
      investigate the effects of 2,4-D in mouse liver on chromosomes as well as 
      hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate 
      dehydrogenase (6PGD), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) 
      which are required for the generation of the pyridine nucleotide pool. The 
      experiments were carried out with a 2,4-D group, an ethanol control for 2,4-D, 
      and saline group for ethanol control group on three generations of mice. Only 
      female parents were given 2,4-D during the gestation period, lactation period and 
      for 33 days following the lactation period. In females of the first cross, 2,4-D 
      caused a significant increase in the activity of LDH, and ethanol alone caused a 
      significant increase in the activities of HK and LDH. In the male offspring of 
      the first cross maternal, 2,4-D caused a significant increase in the activity of 
      LDH, and ethanol alone caused a significant decrease in the activity of 6PGD. In 
      the female offspring of the first cross maternal, ethanol caused a significant 
      increase in the activities of G6PD and MDH. In the female offsprings of the third 
      cross maternal, 2,4-D caused a significant increase in the activity of MDH. No 
      gross morphological changes were observed in internal organs, such as liver, 
      kidney and spleen of the affected animals. Also, a chromosomal study from bone 
      marrow cells indicated no anomalies in chromosomal sets and structures. As a 
      result, 2,4-D had an effect on the first cross maternal and their offsprings. The 
      compound did not affect the parameters studied except MDH enzyme activity in the 
      second and third generation of mice.
FAU - Yilmaz, H Ramazan
AU  - Yilmaz HR
AD  - Suleyman Demirel University, Faculty of Medicine, Department of Medical Biology 
      and Genetics, Isparta, Turkey. hramazany@yahoo.com
FAU - Yuksel, Esref
AU  - Yuksel E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Toxicol Ind Health
JT  - Toxicology and industrial health
JID - 8602702
RN  - 0 (Herbicides)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - 53-59-8 (NADP)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/*toxicity
MH  - Alcohol Oxidoreductases/drug effects/metabolism
MH  - Animals
MH  - Enzyme Activation/drug effects
MH  - Female
MH  - Hepatocytes/drug effects/metabolism
MH  - Herbicides/*toxicity
MH  - Hexokinase/drug effects/metabolism
MH  - Lactation/drug effects
MH  - Liver/*enzymology
MH  - Malate Dehydrogenase/metabolism
MH  - Male
MH  - Maternal Exposure
MH  - Mice
MH  - NADP/drug effects/*metabolism
MH  - Pregnancy
EDAT- 2005/12/14 09:00
MHDA- 2006/02/02 09:00
CRDT- 2005/12/14 09:00
PHST- 2005/12/14 09:00 [pubmed]
PHST- 2006/02/02 09:00 [medline]
PHST- 2005/12/14 09:00 [entrez]
AID - 10.1191/0748233705th231oa [doi]
PST - ppublish
SO  - Toxicol Ind Health. 2005 Oct;21(9):231-7. doi: 10.1191/0748233705th231oa.