PMID- 16348408
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100625
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 57
IP  - 2
DP  - 1991 Feb
TI  - Effects of 2,4-dichlorophenol, a metabolite of a genetically engineered 
      bacterium, and 2,4-dichlorophenoxyacetate on some microorganism-mediated 
      ecological processes in soil.
PG  - 412-8
AB  - A genetically engineered microorganism, Pseudomonas putida PPO301(pRO103), and 
      the plasmidless parent strain, PPO301, were added at approximately 10 CFU/g of 
      soil amended with 500 ppm of 2,4-dichlorophenoxyacetate (2,4-D) (500 mug/g). The 
      degradation of 2,4-D and the accumulation of a single metabolite, identified by 
      gas chromatography-mass spectrophotometry as 2,4-dichlorophenol (2,4-DCP), 
      occurred only in soil inoculated with PPO301(pRO103), wherein 2,4-DCP accumulated 
      to >70 ppm for 5 weeks and the concentration of 2,4-D was reduced to <100 ppm. 
      Coincident with the accumulation of 2,4-DCP was a >400-fold decline in the 
      numbers of fungal propagules and a marked reduction in the rate of CO(2) 
      evolution, whereas 2,4-D did not depress either fungal propagules or respiration 
      of the soil microbiota. 2,4-DCP did not appear to depress the numbers of total 
      heterotrophic, sporeforming, or chitin-utilizing bacteria. In vitro and in situ 
      assays conducted with 2,4-DCP and fungal isolates from the soil demonstrated that 
      2,4-DCP was toxic to fungal propagules at concentrations below those detected in 
      the soil.
FAU - Short, K A
AU  - Short KA
AD  - Environmental Research Laboratory, 200 SW 35th Street, and NSI Technology 
      Services Corp., Environmental Sciences, Environmental Research Laboratory, U.S. 
      Environmental Protection Agency, Corvallis, Oregon 97333; Department of Biology, 
      New York University, New York, New York 10003 ; and Department of Microbiology 
      and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109.
FAU - Doyle, J D
AU  - Doyle JD
FAU - King, R J
AU  - King RJ
FAU - Seidler, R J
AU  - Seidler RJ
FAU - Stotzky, G
AU  - Stotzky G
FAU - Olsen, R H
AU  - Olsen RH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
PMC - PMC182725
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
PMCR- 1991/02/01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
PHST- 1991/02/01 00:00 [pmc-release]
AID - 10.1128/aem.57.2.412-418.1991 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1991 Feb;57(2):412-8. doi: 10.1128/aem.57.2.412-418.1991.