PMID- 16348982
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100625
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 59
IP  - 7
DP  - 1993 Jul
TI  - Effect of X-Prolyl Dipeptidyl Aminopeptidase Deficiency on Lactococcus lactis.
PG  - 2049-55
AB  - The genetic determinant (pepXP) of an X-prolyl dipeptidyl aminopeptidase (PepXP) 
      has recently been cloned and sequenced from both Lactococcus lactis subsp. 
      cremoris (B. Mayo, J. Kok, K. Venema, W. Bockelmann, M. Teuber, H. Reinke, and G. 
      Venema, Appl. Environ. Microbiol. 57:38-44, 1991) and L. lactis subsp. lactis (M. 
      Nardi, M.-C. Chopin, A. Chopin, M.-M. Cals, and J.-C. Gripon, Appl. Environ. 
      Microbiol. 57:45-50, 1991). To examine the possible role of the enzyme in the 
      breakdown of caseins required for lactococci to grow in milk, integration vectors 
      have been constructed and used to specifically inactivate the pepXP gene. After 
      inactivation of the gene in L. lactis subsp. lactis MG1363, which is Lac and Prt, 
      the Lac Prt determinants were transferred by conjugation by using L. lactis 
      subsp. lactis 712 as the donor. Since growth of the transconjugants relative to 
      the PepXP strains was not retarded in milk, it was concluded that PepXP is not 
      essential for growth in that medium. It was also demonstrated that the open 
      reading frame ORF1, upstream of pepXP, was not required for PepXP activity in L. 
      lactis. A marked difference between metenkephalin degradation patterns was 
      observed after incubation of this pentapeptide with cell extracts obtained from 
      wild-type lactococci and pepXP mutants. Therefore, altered expression of the 
      pepXP-encoded general dipeptidyl aminopeptidase activity may change the peptide 
      composition of fermented milk products.
FAU - Mayo, B
AU  - Mayo B
AD  - Department of Genetics, University of Groningen, 9751 NN Haren, The Netherlands, 
      and Institut fur Mikrobiologie, Bundesanstalt fur Milchforchung, D-2300 Kiel 14, 
      Germany.
FAU - Kok, J
AU  - Kok J
FAU - Bockelmann, W
AU  - Bockelmann W
FAU - Haandrikman, A
AU  - Haandrikman A
FAU - Leenhouts, K J
AU  - Leenhouts KJ
FAU - Venema, G
AU  - Venema G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
PMC - PMC182234
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
PMCR- 1993/07/01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 10.1128/aem.59.7.2049-2055.1993 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1993 Jul;59(7):2049-55. doi: 
      10.1128/aem.59.7.2049-2055.1993.