PMID- 16350799
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20161124
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 23
IP  - 5
DP  - 2005 Sep
TI  - [Determination of isosorbide-5-nitrate in human plasma by reversed-phase high 
      performance liquid chromatography].
PG  - 528-30
AB  - The method was established to determine the level of isosorbide-5-nitrate in 
      human plasma by reversed-phase high performance liquid chromatography (RP-HPLC). 
      The sample preparation was carried by alkalizing the plasma sample followed by 
      extraction with dichloromethane. The HPLC analysis was performed under the 
      conditions as follows: a mixture of an aqueous buffer (pH adjusted to 7.8 by 0. 
      03 mol/L ammonia water) and acetonitrile (80: 20, v/v) as mobile phase, 
      paracetamol as the internal standard, and the detection at 230 nm. The linear 
      range was 20 - 1 000 microg/L using the ratio of peak areas; the detection limit 
      was 12 microg/L; the average recovery was (97.11 +/- 2.45)% - (104.34 +/- 2.17)%, 
      the intra-day relative standard deviations (RSDs) were less than 2.52%, and 
      inter-day RSDs were less than 5.21%.
FAU - He, Lin
AU  - He L
AD  - Laboratory of Clinical Pharmacology, Sichuan Provincial People's Hospital, 
      Chengdu 610072, China. helinn@tom.com
FAU - Yu, Jiying
AU  - Yu J
FAU - Wu, Zhengzhong
AU  - Wu Z
FAU - Sun, Shiming
AU  - Sun S
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - IA7306519N (Isosorbide Dinitrate)
RN  - LX1OH63030 (isosorbide-5-mononitrate)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Isosorbide Dinitrate/*analogs & derivatives/blood
MH  - Reproducibility of Results
EDAT- 2005/12/15 09:00
MHDA- 2010/05/07 06:00
CRDT- 2005/12/15 09:00
PHST- 2005/12/15 09:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PHST- 2005/12/15 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2005 Sep;23(5):528-30.