PMID- 16351713
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20181113
IS  - 1471-2350 (Electronic)
IS  - 1471-2350 (Linking)
VI  - 6
DP  - 2005 Dec 13
TI  - The host response to the probiotic Escherichia coli strain Nissle 1917: specific 
      up-regulation of the proinflammatory chemokine MCP-1.
PG  - 43
AB  - BACKGROUND: The use of live microorganisms to influence positively the course of 
      intestinal disorders such as infectious diarrhea or chronic inflammatory 
      conditions has recently gained increasing interest as a therapeutic alternative. 
      In vitro and in vivo investigations have demonstrated that probiotic-host 
      eukaryotic cell interactions evoke a large number of responses potentially 
      responsible for the effects of probiotics. The aim of this study was to improve 
      our understanding of the E. coli Nissle 1917-host interaction by analyzing the 
      gene expression pattern initiated by this probiotic in human intestinal 
      epithelial cells. METHODS: Gene expression profiles of Caco-2 cells treated with 
      E. coli Nissle 1917 were analyzed with microarrays. A second human intestinal 
      cell line and also pieces of small intestine from BALB/c mice were used to 
      confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead 
      array (CBA) to detect secretion of corresponding proteins. RESULTS: Whole genome 
      expression analysis revealed 126 genes specifically regulated after treatment of 
      confluent Caco-2 cells with E. coli Nissle 1917. Among others, expression of 
      genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 
      ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2alpha) and 
      macrophage inflammatory protein-2 beta (MIP-2beta) was increased up to 10 fold. 
      Caco-2 cells cocultured with E. coli Nissle 1917 also secreted high amounts of 
      MCP-1 protein. Elevated levels of MCP-1 and MIP-2alpha mRNA could be confirmed 
      with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal 
      tissue. CONCLUSION: Thus, probiotic E. coli Nissle 1917 specifically upregulates 
      expression of proinflammatory genes and proteins in human and mouse intestinal 
      epithelial cells.
FAU - Ukena, Sya N
AU  - Ukena SN
AD  - German Research Centre for Biotechnology, Mucosal Immunity Group, Mascheroder Weg 
      1, 38124 Braunschweig, Germany. suk@gbf.de
FAU - Westendorf, Astrid M
AU  - Westendorf AM
FAU - Hansen, Wiebke
AU  - Hansen W
FAU - Rohde, Manfred
AU  - Rohde M
FAU - Geffers, Robert
AU  - Geffers R
FAU - Coldewey, Sina
AU  - Coldewey S
FAU - Suerbaum, Sebastian
AU  - Suerbaum S
FAU - Buer, Jan
AU  - Buer J
FAU - Gunzer, Florian
AU  - Gunzer F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051213
PL  - England
TA  - BMC Med Genet
JT  - BMC medical genetics
JID - 100968552
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Monokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Biological Therapy/*methods
MH  - Caco-2 Cells
MH  - Chemokine CCL2/*genetics
MH  - Chemokine CXCL2
MH  - Escherichia coli/cytology/*immunology
MH  - Gene Expression Profiling
MH  - Humans
MH  - *Immunotherapy
MH  - Inflammation/genetics
MH  - Intestinal Diseases/therapy
MH  - Intestines/cytology/immunology
MH  - Monokines/genetics
MH  - *Probiotics
MH  - RNA, Messenger/analysis
MH  - Up-Regulation/immunology
PMC - PMC1326229
EDAT- 2005/12/15 09:00
MHDA- 2006/01/21 09:00
PMCR- 2005/12/13
CRDT- 2005/12/15 09:00
PHST- 2005/07/27 00:00 [received]
PHST- 2005/12/13 00:00 [accepted]
PHST- 2005/12/15 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/12/15 09:00 [entrez]
PHST- 2005/12/13 00:00 [pmc-release]
AID - 1471-2350-6-43 [pii]
AID - 10.1186/1471-2350-6-43 [doi]
PST - epublish
SO  - BMC Med Genet. 2005 Dec 13;6:43. doi: 10.1186/1471-2350-6-43.