PMID- 16352389
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20220331
IS  - 0301-2115 (Print)
IS  - 0301-2115 (Linking)
VI  - 126
IP  - 1
DP  - 2006 May 1
TI  - Combination of calcium ionophore A23187 with puromycin salvages human 
      unfertilized oocytes after ICSI.
PG  - 72-6
AB  - OBJECTIVE: To determine whether oocyte activation using a combination of calcium 
      ionophore A23187 (A23187) with puromycin could salvage human unfertilized oocytes 
      after ICSI. STUDY DESIGN: One hundred and thirteen discarded unfertilized oocytes 
      20-68 h after ICSI were assigned to four groups: ICSI 20-h group, ICSI 44-h 
      group, ICSI 68-h group and control. All unfertilized oocytes were exposed to 
      A23187 (5 microM) for 5 min and subsequently were incubated with puromycin (10 
      microg/ml) for 4 h. Sex chromosomal analysis was performed by dual color 
      fluorescence in situ hybridization (FISH). RESULTS: The combination of A23187 
      with puromycin could activate the unfertilized oocytes 20-68 h after ICSI. The 
      best results were achieved in the ICSI 20-h group, which exhibited an activation 
      rate of 91.2% (31/34), a cleavage rate of 64.7% (22/34) and 44.1% (15/34) 
      high-quality embryos. The activation rate, cleavage rate and the number of 
      high-quality embryos appeared to decrease with the cultured time of unfertilized 
      oocytes after ICSI. FISH analysis showed six embryos with XX and seven embryos 
      with XY in 16 embryos derived from 2PN2PB. CONCLUSIONS: The combination of 
      calcium ionophore A23187 with puromycin could effectively salvage unfertilized 
      oocytes within 20 h after ICSI.
FAU - Lu, Qun
AU  - Lu Q
AD  - Reproductive Medical Center, Shandong Provincial Hospital, Shandong University, 
      Jinan 250021, China.
FAU - Zhao, Yueran
AU  - Zhao Y
FAU - Gao, Xuan
AU  - Gao X
FAU - Li, Yuan
AU  - Li Y
FAU - Ma, Shuiying
AU  - Ma S
FAU - Mullen, Steve
AU  - Mullen S
FAU - Critser, J K
AU  - Critser JK
FAU - Chen, Zi-Jiang
AU  - Chen ZJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051213
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
RN  - 0 (Ionophores)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 4A6ZS6Q2CL (Puromycin)
SB  - IM
MH  - Calcimycin/*pharmacology
MH  - Fertilization
MH  - *Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ionophores/*pharmacology
MH  - Oocytes/*drug effects/physiology
MH  - Puromycin/*pharmacology
MH  - *Sperm Injections, Intracytoplasmic
MH  - Time Factors
MH  - Treatment Failure
EDAT- 2005/12/15 09:00
MHDA- 2006/12/09 09:00
CRDT- 2005/12/15 09:00
PHST- 2005/01/20 00:00 [received]
PHST- 2005/09/09 00:00 [revised]
PHST- 2005/10/29 00:00 [accepted]
PHST- 2005/12/15 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2005/12/15 09:00 [entrez]
AID - S0301-2115(05)00609-3 [pii]
AID - 10.1016/j.ejogrb.2005.10.038 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2006 May 1;126(1):72-6. doi: 
      10.1016/j.ejogrb.2005.10.038. Epub 2005 Dec 13.