PMID- 16352395
OWN - NLM
STAT- MEDLINE
DCOM- 20060425
LR  - 20060227
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 396
IP  - 2
DP  - 2006 Mar 27
TI  - Increased spinal cord NGF levels in rats with cobalamin (vitamin B12) deficiency.
PG  - 153-8
AB  - We have recently demonstrated that the neuropathological morphological 
      alterations caused by cobalamin (Cbl) deficiency in the rat central nervous 
      system are related to the vitamin's inability to modulate the synthesis of some 
      neurotoxic and neurotrophic agents in opposite directions. In the present study, 
      we measured nerve growth factor (NGF) levels in the spinal cord (SC) and 
      cerebrospinal fluid (CSF) of rats made Cbl-deficient (Cbl-D) by means of total 
      gastrectomy (TG) or a Cbl-D diet. In both cases, Cbl deficiency increased SC and 
      CSF NGF levels after the appearance of myelinolytic lesions in the SC white 
      matter (SCWM) (i.e. after the second post-TG month), and these changes were 
      normalised by Cbl treatment in the 4-month-totally-gastrectomised (TGX) rats. 
      Intracerebroventricular (i.c.v.) anti-NGF-antibody treatment prevented the onset 
      of the myelinolytic SCWM lesions in the 2-month-TGX rats (i.e. when SC and CSF 
      NGF levels are still normal) and normalised the ultrastructure of the SCWM in the 
      4-month-TGX rats, which was however worsened by the i.c.v. administration of NGF. 
      These findings demonstrate that: (i) Cbl deficiency increases SC and CSF NGF 
      levels; and (ii) endogenous NGF seems to play a noxious role in the progression 
      of rat Cbl-D central neuropathy.
FAU - Scalabrino, Giuseppe
AU  - Scalabrino G
AD  - Institute of General Pathology, University of Milan, Via Mangiagalli 31, 20133 
      Milano, Italy. giuseppe.scalabrino@unimi.it
FAU - Mutti, Elena
AU  - Mutti E
FAU - Veber, Daniela
AU  - Veber D
FAU - Aloe, Luigi
AU  - Aloe L
FAU - Corsi, Massimiliano M
AU  - Corsi MM
FAU - Galbiati, Stefania
AU  - Galbiati S
FAU - Tredici, Giovanni
AU  - Tredici G
LA  - eng
PT  - Journal Article
DEP - 20051213
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Male
MH  - Nerve Degeneration/cerebrospinal fluid/etiology/*metabolism/*pathology
MH  - Nerve Growth Factors/cerebrospinal fluid/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*metabolism/*pathology
MH  - Vitamin B 12 Deficiency/cerebrospinal fluid/complications/*metabolism/*pathology
EDAT- 2005/12/15 09:00
MHDA- 2006/04/28 09:00
CRDT- 2005/12/15 09:00
PHST- 2005/10/14 00:00 [received]
PHST- 2005/11/08 00:00 [revised]
PHST- 2005/11/13 00:00 [accepted]
PHST- 2005/12/15 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2005/12/15 09:00 [entrez]
AID - S0304-3940(05)01314-5 [pii]
AID - 10.1016/j.neulet.2005.11.029 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 Mar 27;396(2):153-8. doi: 10.1016/j.neulet.2005.11.029. Epub 
      2005 Dec 13.