PMID- 16352404
OWN - NLM
STAT- MEDLINE
DCOM- 20060718
LR  - 20071114
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 113
IP  - 3-4
DP  - 2006 Mar 31
TI  - Functional analysis of bovine herpesvirus 1 (BHV-1) genes expressed during 
      latency.
PG  - 199-210
AB  - Bovine herpes virus 1 (BHV-1) establishes latency in sensory neurons of 
      trigeminal ganglia (TG), and germinal centers of pharyngeal tonsil. Periodically 
      BHV-1 reactivates from latency, virus is shed, and consequently virus 
      transmission occurs. Two transcripts, the latency related (LR) RNA and ORF-E, are 
      abundantly expressed in TG of latently infected cattle. A LR mutant strain of 
      BHV-1 was constructed that contains stop codons near the beginning of the LR-RNA. 
      The LR mutant virus does not express two proteins encoded by the LR gene, or 
      reactivate from latency suggesting that LR protein expression regulates the 
      latency-reactivation cycle. Higher levels of apoptosis occur in TG of calves 
      infected with the LR mutant versus wild type BHV-1 indicating that the 
      anti-apoptotic properties of the LR gene regulate the latency-reactivation cycle. 
      The LR gene also inhibits bICP0 expression and mammalian cell growth, but these 
      functions do not require LR protein expression. In contrast, the ability of the 
      LR gene to inhibit apoptosis appears to require LR protein expression. A small 
      open reading frame (ORF-E) that is located within the LR promoter is expressed in 
      the nucleus of neuroblastoma cells. We predict that the LR gene and ORF-E 
      regulate the BHV-1 latency-reactivation cycle.
FAU - Jones, C
AU  - Jones C
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      and School of Biological Sciences, University of Nebraska, Lincoln, NE 68583, 
      USA. cjones@unlnotes.unl.edu
FAU - Geiser, V
AU  - Geiser V
FAU - Henderson, G
AU  - Henderson G
FAU - Jiang, Y
AU  - Jiang Y
FAU - Meyer, F
AU  - Meyer F
FAU - Perez, S
AU  - Perez S
FAU - Zhang, Y
AU  - Zhang Y
LA  - eng
GR  - 1P20RR15635/RR/NCRR NIH HHS/United States
GR  - 1T32 AI060547/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
DEP - 20051213
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cattle
MH  - Cattle Diseases/*virology
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Herpesvirus 1, Bovine/*genetics/pathogenicity/physiology
MH  - *Mutation
MH  - Trigeminal Ganglion/virology
MH  - Viral Proteins/genetics/metabolism
MH  - Virus Activation
MH  - Virus Latency/*genetics
RF  - 118
EDAT- 2005/12/15 09:00
MHDA- 2006/07/19 09:00
CRDT- 2005/12/15 09:00
PHST- 2005/12/15 09:00 [pubmed]
PHST- 2006/07/19 09:00 [medline]
PHST- 2005/12/15 09:00 [entrez]
AID - S0378-1135(05)00368-8 [pii]
AID - 10.1016/j.vetmic.2005.11.009 [doi]
PST - ppublish
SO  - Vet Microbiol. 2006 Mar 31;113(3-4):199-210. doi: 10.1016/j.vetmic.2005.11.009. 
      Epub 2005 Dec 13.