PMID- 16357492
OWN - NLM
STAT- MEDLINE
DCOM- 20060502
LR  - 20140729
IS  - 0030-3747 (Print)
IS  - 0030-3747 (Linking)
VI  - 38
IP  - 2
DP  - 2006
TI  - Frequency of chromosome 17 aneuploidy in primary and recurrent pterygium by 
      interphase-fluorescence in situ hybridization.
PG  - 89-94
AB  - AIM: To investigate chromosome 17 numerical aberrations by using fluorescence in 
      situ hybridization (FISH) in pterygia and to find out whether there is any 
      association between chromosome 17 aneuploidy and recurrent pterygia. METHODS: 
      Pterygium tissue samples were taken from 21 patients by surgical excision. 
      Eighteen of them had primary and 3 had recurrent pterygium. Peripheral whole 
      blood interphase cells obtained from 11 healthy subjects were assigned as control 
      group. The cells from pterygium tissue and peripheral blood were incubated with a 
      hypotonic solution and fixed in order to obtain interphase nuclei. FISH analysis 
      with chromosome-17-specific alpha-satellite DNA probe was performed on both the 
      interphase nuclei of pterygium tissue (of patients) and peripheral whole blood 
      cells of controls. RESULTS: The mean percentage of chromosome 17 aneuploidy was 
      4.71% for the pterygia group and 4.41% for the controls. No significant 
      difference of chromosome 17 aneuploidy was observed between the patients and the 
      controls. When the group of patients with recurrences was compared with the group 
      without recurrences, there was a significant difference in the frequency of 
      chromosome 17 aneuploidy (U = 17, p = 0.029). CONCLUSIONS: Chromosome 17 
      aneuploidy is probably not an important factor in the formation of pterygium, but 
      it may be related to recurrence.
FAU - Kamis, Umit
AU  - Kamis U
AD  - Department of Ophthalmology, Faculty of Medicine, Selcuk University, Konya, 
      Turkey. umitkamis@hotmail.com
FAU - Kerimoglu, Hurkan
AU  - Kerimoglu H
FAU - Ozkagnici, Ahmet
AU  - Ozkagnici A
FAU - Acar, Hasan
AU  - Acar H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051215
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - DNA/*analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Pterygium/*genetics
MH  - Recurrence
MH  - Retrospective Studies
EDAT- 2005/12/17 09:00
MHDA- 2006/05/04 09:00
CRDT- 2005/12/17 09:00
PHST- 2005/02/18 00:00 [received]
PHST- 2005/06/22 00:00 [accepted]
PHST- 2005/12/17 09:00 [pubmed]
PHST- 2006/05/04 09:00 [medline]
PHST- 2005/12/17 09:00 [entrez]
AID - 90329 [pii]
AID - 10.1159/000090329 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2006;38(2):89-94. doi: 10.1159/000090329. Epub 2005 Dec 15.