PMID- 16358687
OWN - NLM
STAT- MEDLINE
DCOM- 20100420
LR  - 20191210
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 20
IP  - 5
DP  - 2002 Sep
TI  - [Determination of amino acids and glucose in amino acid injection by anion 
      exchange chromatography with integrated pulsed amperometric detection].
PG  - 398-402
AB  - A direct, sensitive, simple and practical method for the determination of amino 
      acids and glucose in amino acid injection by anion exchange chromatography with 
      integrated pulsed amperometric detection was developed. There is no need for 
      sample pretreatment. The retention behavior of amino acids and glucose on the 
      anion exchange column was investigated using sodium hydroxide and sodium acetate 
      as eluent. When the concentration of sodium hydroxide was changed, the change in 
      the retention times was generally greater for amino acids than for glucose. The 
      difference can be used to improve the separation between amino acids and glucose. 
      When the concentration of sodium acetate was changed, the change in the retention 
      times was generally greater for amino acids containing two carboxyl groups than 
      for amino acids containing only one carboxyl group. The difference can be used to 
      improve the separation between di-carboxylic amino acids and mono-carboxylic 
      amino acids. In order to separate amino acids and glucose, a ternary gradient 
      elution method with water, sodium hydroxide and sodium acetate as mobile phases 
      was employed. The optimized gradient elution condition for the analysis of 17 
      amino acids and glucose in amino acid injection was obtained. The time of the 
      gradient elution program was 74 min. Under the optimized conditions and the 
      column temperature of 30 degrees C, the detection limits for 17 amino acids and 
      glucose were 0.3 pmol- 10.3 pmol. The calibration graphs of peak area for all the 
      analytes were linear in the ranges about two orders of magnitude. The relative 
      standard deviations (RSDs) (n = 5) were 0.7% - 3.8%. The recoveries of added 
      standard were 88.3% - 104.6%.
FAU - Yu, Hong
AU  - Yu H
AD  - Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, 
      Beijing 100085, China.
FAU - Ding, Yong-Sheng
AU  - Ding YS
FAU - Mou, Shi-Fen
AU  - Mou SF
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Amino Acids)
RN  - 0 (Anion Exchange Resins)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Amino Acids/*analysis
MH  - Anion Exchange Resins/*chemistry
MH  - Chromatography, Ion Exchange/instrumentation/*methods
MH  - Glucose/*analysis
EDAT- 2005/12/20 09:00
MHDA- 2010/04/21 06:00
CRDT- 2005/12/20 09:00
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2010/04/21 06:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2002 Sep;20(5):398-402.