PMID- 16359687
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1118
IP  - 1
DP  - 2006 Jun 16
TI  - Optimizing the integrated pulsed amperometric multicycle step waveform for the 
      determination of tetracyclines.
PG  - 35-40
AB  - A method of modified integrated pulsed amperometric detection with multicycle 
      step waveform (Multi-IPAD) following high-performance liquid chromatography 
      (HPLC) was applied for the determination of tetracyclines (TCs) including 
      dimethyltetracycline (DMTC), oxytetracycline (OTC) and tetracycline (TC). The key 
      advantages of the Multi-IPAD are the abilities to enhance sensitivity and 
      reproducibility and the ability to keep working electrode clean through the use 
      of a high-frequent waveform alteration in integration step and the use of a 
      cleaning potential, which is quite different from conventional three-step 
      potential waveform. The analyses were carried out using the mobile phase of 
      acetonitrile-water mixture solution (10:90, v/v) containing 1% perchloric acid on 
      a C(18) column at a flow rate of 0.21 mL/min. The IPAD waveform parameters were 
      optimized to maximize the signal-to-noise ratio (S/N) and successfully applied 
      for the sensitive detection of TCs. The detection limits (S/N=3, 20 microL 
      injected) were 0.07 mg/L for DMTC, 0.08 mg/L for OTC and 0.05 mg/L for TC. The 
      peak height relative standard deviations (RSDs) of every compound for replicate 
      injection (n=15) determined were below 4.6%.
FAU - Cai, Yu-e
AU  - Cai YE
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Cai, Yaqi
AU  - Cai Y
FAU - Shi, Yali
AU  - Shi Y
FAU - Mou, Shifen
AU  - Mou S
FAU - Lu, Yiqiang
AU  - Lu Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051215
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Tetracyclines)
RN  - X20I9EN955 (Oxytetracycline)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Electrochemistry/*methods
MH  - Oxytetracycline/analysis
MH  - Reproducibility of Results
MH  - Tetracyclines/*analysis
EDAT- 2005/12/20 09:00
MHDA- 2006/08/25 09:00
CRDT- 2005/12/20 09:00
PHST- 2005/08/31 00:00 [received]
PHST- 2005/11/14 00:00 [revised]
PHST- 2005/11/14 00:00 [accepted]
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
AID - S0021-9673(05)02204-1 [pii]
AID - 10.1016/j.chroma.2005.11.091 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Jun 16;1118(1):35-40. doi: 10.1016/j.chroma.2005.11.091. 
      Epub 2005 Dec 15.