PMID- 16360933
OWN - NLM
STAT- MEDLINE
DCOM- 20060310
LR  - 20061115
IS  - 0944-7113 (Print)
IS  - 0944-7113 (Linking)
VI  - 13
IP  - 1-2
DP  - 2006 Jan
TI  - Inhibitory effects of aloe carboxypeptidase fraction on streptozotocin-induced 
      enhancement of vascular permeability in the pancreatic islets.
PG  - 49-60
AB  - The protective actions of components isolated from Aloe arborescens Miller var. 
      natalensis Berger (Kidachi aloe in Japanese) on streptozotocin (Sz)-induced 
      necrosis of B cells in the pancreatic islets of the mouse were investigated to 
      clarify its action mechanism involved in anti-diabetic effects. In this 
      experiment, phenol low molecular weight components of aloin and aloin A that were 
      anti-oxidants and derived from the leaf skin or pulp extract, an aloe 
      carboxypeptidase fraction that is a inhibitor of enhanced vascular permeability 
      and a glycoprotein component that decreases blood glucose were tested with mice 
      precedently administered with Sz which is known as a cytotoxin specific to B 
      cells. The results showed that the treatment group receiving Sz followed by the 
      aloe carboxypeptidase fraction increased the inhibition of dye leakage by 75.8% 
      (p<0.001) in the extract of whole pancreas in comparison to the control group and 
      the aloe carboxypeptidase fraction group also increased the inhibition effect by 
      68.4% (p<0.001) in the extract of pancreatic islets as compared to the control 
      group. The carboxypeptidase is an aloe-derived protease known to inhibit the 
      acetic acid-related enhancement of intraperitoneal vascular permeability in mice. 
      Further, the elevation of blood glucose in Sz-induced diabetic mice 
      intraperitoneally given the aloe carboxypeptitase fraction was significantly 
      (p<0.01-0.001) restrained at 3, 7 and 14 days after the injection as compared to 
      the control group given solvent only. The results of this experiment suggested 
      that the inhibitory effect on the enhancement of vascular permeability related to 
      the vascular acute inflammatory response at Sz-induced lesions of pancreatic 
      islets was involved in the action mechanism of this enzyme.
FAU - Beppu, H
AU  - Beppu H
AD  - Fujita Memorial Institute of Pharmacognosy, Fujita Health University, 1865 
      Isshiki-cho, Hisai, Mie 514-1296, Japan. hbeppu@fujita-hu.ac.jp
FAU - Shimpo, K
AU  - Shimpo K
FAU - Chihara, T
AU  - Chihara T
FAU - Tamai, I
AU  - Tamai I
FAU - Nomoto-Yamaji, S
AU  - Nomoto-Yamaji S
FAU - Ozaki, S
AU  - Ozaki S
FAU - Ito, S
AU  - Ito S
FAU - Kuzuya, H
AU  - Kuzuya H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050818
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Blood Glucose)
RN  - 0 (Plant Extracts)
RN  - EC 3.4.- (Carboxypeptidases)
SB  - IM
MH  - Aloe/*enzymology
MH  - Animals
MH  - Blood Glucose
MH  - Capillary Permeability/*drug effects
MH  - Carboxypeptidases/metabolism/*pharmacology
MH  - Diabetes Mellitus, Experimental/*drug therapy/*physiopathology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Islets of Langerhans/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Plant Extracts/metabolism/pharmacology
MH  - Plant Leaves/enzymology
MH  - Time Factors
EDAT- 2005/12/20 09:00
MHDA- 2006/03/11 09:00
CRDT- 2005/12/20 09:00
PHST- 2004/04/20 00:00 [received]
PHST- 2004/06/25 00:00 [accepted]
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2006/03/11 09:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
AID - S0944-7113(05)00112-1 [pii]
AID - 10.1016/j.phymed.2004.06.026 [doi]
PST - ppublish
SO  - Phytomedicine. 2006 Jan;13(1-2):49-60. doi: 10.1016/j.phymed.2004.06.026. Epub 
      2005 Aug 18.