PMID- 16361427
OWN - NLM
STAT- MEDLINE
DCOM- 20060316
LR  - 20201006
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 1
DP  - 2006 Jan
TI  - Screening for CD8 cytotoxic T lymphocytes specific for Gag of human 
      immunodeficiency virus type 1 subtype B' Henan isolate from China and 
      identification of novel epitopes restricted by the HLA-A2 and HLA-A11 alleles.
PG  - 151-158
LID - 10.1099/vir.0.81335-0 [doi]
AB  - The human immunodeficiency virus type 1 (HIV-1) epidemic in China is increasing 
      rapidly at an irrepressible rate. It is caused by HIV-1 subtype B' in central 
      China. After the full-length genome sequencing of the Henan isolate was 
      performed, the definition of optimal cytotoxic T-lymphocyte (CTL) epitopes across 
      the Henan isolate genome has become crucial for vaccine design. In this study, by 
      using ELISPOT assays with synthetic peptides corresponding to the sequence of the 
      Henan isolate, the identification and analysis of Gag-specific CTL responses 
      among 28 treated and 26 untreated infected paid blood donors (PBDs) from the 
      Henan and Hubei provinces of China are presented. These studies focused on CTL 
      responses restricted by the human leukocyte antigen (HLA)-A2 and -A11 molecules, 
      two of the most prominent HLA-A alleles in the Chinese population. The results 
      suggested that, in the subgroup analysis, the magnitude of response in the 
      infected treated subgroup [median, 93 spot-forming cells (SFCs) per 10(6) 
      peripheral blood mononuclear cells (PBMCs)] was significantly lower than that in 
      the chronically infected untreated subgroup (median, 221 SFCs per 10(6) PBMCs), 
      and HLA-A2-restricted treated PBDs had a response of a much higher frequency and 
      magnitude than that of HLA-A11-restricted treated PBDs. Moreover, some novel 
      peptides restricted by the HLA-A2 and -A11 molecules were identified.
FAU - Gong, Xiaoyan
AU  - Gong X
AD  - Modern Virology Research Center (MVRC), State Key Laboratory of Virology, College 
      of Life Sciences, Wuhan University, Wuhan 430072, China.
FAU - Gui, Xien
AU  - Gui X
AD  - Zhongnan Hospital, Wuhan University, Wuhan 430072, China.
FAU - Zhang, Yuxia
AU  - Zhang Y
AD  - Molecular Virology Department (MVD), Institute of Microbiology, Chinese Academy 
      of Sciences, Beijing 100080, China.
FAU - Tien, Po
AU  - Tien P
AD  - Molecular Virology Department (MVD), Institute of Microbiology, Chinese Academy 
      of Sciences, Beijing 100080, China.
AD  - Modern Virology Research Center (MVRC), State Key Laboratory of Virology, College 
      of Life Sciences, Wuhan University, Wuhan 430072, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Gene Products, gag)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A11 Antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Immunodominant Epitopes)
SB  - IM
MH  - Alleles
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - China/epidemiology
MH  - Gene Products, gag/genetics/*immunology
MH  - HIV Infections/blood/*immunology
MH  - HIV Seropositivity/*immunology
MH  - HIV-1/*immunology
MH  - HLA-A Antigens/*genetics/immunology
MH  - HLA-A11 Antigen
MH  - HLA-A2 Antigen/*genetics/immunology
MH  - Humans
MH  - Immunodominant Epitopes/*analysis/immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
EDAT- 2005/12/20 09:00
MHDA- 2006/03/17 09:00
CRDT- 2005/12/20 09:00
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2006/03/17 09:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
AID - 10.1099/vir.0.81335-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Jan;87(Pt 1):151-158. doi: 10.1099/vir.0.81335-0.