PMID- 16361491
OWN - NLM
STAT- MEDLINE
DCOM- 20061019
LR  - 20200403
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 84
IP  - 1
DP  - 2006 Jan
TI  - An investigation of susceptibility to porcine reproductive and respiratory 
      syndrome virus between two genetically diverse commercial lines of pigs.
PG  - 49-57
AB  - The objective of this study was to determine whether host genetics play a role in 
      susceptibility to the respiratory disease in growing pigs caused by the porcine 
      reproductive and respiratory syndrome virus (PRRSV). Based on a previous study, 2 
      genetically diverse commercial lines of pigs that also were divergent in the 
      susceptibility of monocyte-derived macrophages to PRRSV infection in vitro were 
      selected for an in vivo challenge study. Based on the average percentage of 
      infected macrophages for each line, a line derived from the Large White breed was 
      characterized as fluorescence-activated cell sorting(hi) (FACS(hi)), and a line 
      derived from Duroc and Pietrain breeds was characterized as FACS(lo). Pigs from 
      each line were challenged at 6 wk of age with PRRSV VR-2385 and necropsied at 10 
      or 21 d after infection. Data collected included clinical evaluation of disease, 
      virus titration in serum and lung lavage fluid, macroscopic lung lesion scores, 
      and microscopic lung lesion scores. The FACS(lo) line had consistently more 
      severe clinical disease compared with the FACS(hi) line in the early stages of 
      infection. Differences between line means were significant (P < 0.05) at 10 d 
      after infection for all variables just described, and the FACS(lo) line showed 
      more severe signs of disease. By 21 d after infection, clinical signs and lesions 
      were resolving, and the differences between lines were significant (P < 0.04) 
      only for microscopic lung lesion scores but approached significance (P < 0.08) 
      for virus titer in serum. At 21 d after infection, the relationship between the 
      lines reversed; the FACS(hi) line had higher serum virus titers than the FACS(lo) 
      line. This report provides evidence that strongly suggests the existence of a 
      host genetic component in disease susceptibility to PRRSV and indicates that 
      further study is warranted to define the cellular mechanisms that affect disease 
      susceptibility.
FAU - Vincent, A L
AU  - Vincent AL
AD  - Department of Veterinary Microbiology and Preventive Medicine, Iowa State 
      University, Ames, 50011, USA.
FAU - Thacker, B J
AU  - Thacker BJ
FAU - Halbur, P G
AU  - Halbur PG
FAU - Rothschild, M F
AU  - Rothschild MF
FAU - Thacker, E L
AU  - Thacker EL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
SB  - IM
MH  - Animals
MH  - *Genetic Predisposition to Disease
MH  - Genetic Variation
MH  - Lung/pathology
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/*virology
MH  - Swine/classification/genetics/virology
PMC - PMC7110373
EDAT- 2005/12/20 09:00
MHDA- 2006/10/20 09:00
PMCR- 2020/04/01
CRDT- 2005/12/20 09:00
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2006/10/20 09:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
PHST- 2020/04/01 00:00 [pmc-release]
AID - 84/1/49 [pii]
AID - 10.2527/2006.84149x [doi]
PST - ppublish
SO  - J Anim Sci. 2006 Jan;84(1):49-57. doi: 10.2527/2006.84149x.