PMID- 16362500
OWN - NLM
STAT- MEDLINE
DCOM- 20060106
LR  - 20181113
IS  - 0022-2631 (Print)
IS  - 0022-2631 (Linking)
VI  - 205
IP  - 3
DP  - 2005 Jun
TI  - N-methyl-D-aspartate receptor in human prostate cancer.
PG  - 125-8
AB  - Expression of the N-methyl-D-aspartate receptor (NMDAr) and its involvement in 
      cellular proliferation is well-known in tumors of neuronal tissue, such as glioma 
      and neuroblastoma. We have investigated NMDAr expression in the normal, 
      hyperplastic and neoplastic human prostate by immunohistochemistry. Low stromal 
      NMDAr immunostaining was observed in 2 of 12 (17%) normal prostate specimens, but 
      epithelial NMDAr staining was not seen. Of 18 benign prostatic hyperplasia (BPH) 
      specimens, none had stromal NMDAr staining, but 2 had low and 1 had high 
      epithelial NMDAr immunoreactivity. Moderate to high NMDAr immunostaining was 
      observed in the stroma of 60 of 145 (41%) prostate cancer (PCa) specimens. 
      Epithelial NMDAr staining was low in 26 (18%) and moderate to high in 36 (25%) of 
      145 PCa specimens. We have also examined the effects of the NMDAr antagonist 
      memantine on the growth of ten human cancer cell lines: four prostate, two breast 
      and four colon. The NMDAr antagonist memantine inhibited in-vitro growth of all 
      ten cell lines, with half-maximal growth-inhibition at 5 to 20 microg/ml (23 to 
      92 microM) memantine. An NMDA agonist, L-cysteinesulfinic acid, stimulated 
      cellular proliferation of all ten cell lines, with maximal growth-stimulation 
      (30% to 75%, depending on the cell line) observed between doses of 33 to 66 
      microM. Our data provide evidence for the expression and activity of NMDAr in 
      prostate cancer.
FAU - Abdul, M
AU  - Abdul M
AD  - Edward Via Virginia College of Osteopathic Medicine and Virginia Polytechnic 
      Institute and State University, Blacksburg, Virginia, USA.
FAU - Hoosein, N
AU  - Hoosein N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Membr Biol
JT  - The Journal of membrane biology
JID - 0211301
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - K848JZ4886 (Cysteine)
RN  - UZY4HYK4ZX (cysteine sulfinic acid)
RN  - W8O17SJF3T (Memantine)
SB  - IM
MH  - Breast/metabolism
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Colonic Neoplasms
MH  - Cysteine/analogs & derivatives/pharmacology
MH  - Dizocilpine Maleate/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Memantine/pharmacology
MH  - Prostate/physiology
MH  - Prostatic Hyperplasia/physiopathology
MH  - Prostatic Neoplasms/*physiopathology
MH  - Receptors, N-Methyl-D-Aspartate/*physiology
EDAT- 2005/12/20 09:00
MHDA- 2006/01/07 09:00
CRDT- 2005/12/20 09:00
PHST- 2005/09/09 00:00 [received]
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2006/01/07 09:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
AID - 10.1007/s00232-005-0777-0 [doi]
PST - ppublish
SO  - J Membr Biol. 2005 Jun;205(3):125-8. doi: 10.1007/s00232-005-0777-0.