PMID- 16362971
OWN - NLM
STAT- MEDLINE
DCOM- 20060619
LR  - 20231213
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 73
IP  - 3
DP  - 2006 Mar
TI  - Spetex-1: a new component in the middle piece of flagellum in rodent spermatozoa.
PG  - 342-9
AB  - Spetex-1 has recently been isolated by differential display and screening of cDNA 
      library. It encodes a protein of 556 amino acid residues possessing coiled-coil 
      motifs. In the rat seminiferous tubules (ST), Spetex-1 was expressed in the 
      cytoplasm of elongating spermatids. To examine the subcellular distribution of 
      Spetex-1 in mature spermatozoa, we performed biochemical and immunocytochemical 
      approaches. We found that Spetex-1 that was synthesized in the cytoplasm of 
      elongating spermatids was subsequently integrated as a middle piece component 
      into spermatozoa during spermiogenesis. After integration, the majority of 
      Spetex-1 in spermatozoa could be extracted by 6M urea under reduced condition but 
      not released by the treatment of 1% Triton X-100. Immunoelectron microscopy 
      demonstrated that Spetex-1 seemed to locate at the inner side of outer dense 
      fibers (ODFs) in the middle piece or the narrow space between ODFs and axoneme. 
      Spetex-1 might be involved in the stability of the structural complexity 
      comprising axoneme and ODFs in the middle piece of sperm flagellum.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Iida, Hiroshi
AU  - Iida H
AD  - Laboratory of Zoology, Graduate School of Agriculture, Kyushu University, 
      Higashiku Hakozaki 6-10-1, Fukuoka, Japan.
FAU - Honda, Yoshiko
AU  - Honda Y
FAU - Matsuyama, Takuya
AU  - Matsuyama T
FAU - Shibata, Yosaburo
AU  - Shibata Y
FAU - Inai, Tetsuichiro
AU  - Inai T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Proteins)
RN  - 0 (Spata18 protein, rat)
RN  - 0 (SPATA18 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cytoplasm/chemistry
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Microscopy, Immunoelectron/methods
MH  - Mitochondrial Proteins
MH  - Proteins/*chemistry/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sensitivity and Specificity
MH  - Sperm Tail/*chemistry
MH  - Spermatogenesis/genetics/physiology
MH  - Spermatozoa/*physiology
MH  - Testis/physiology
EDAT- 2005/12/20 09:00
MHDA- 2006/06/20 09:00
CRDT- 2005/12/20 09:00
PHST- 2005/12/20 09:00 [pubmed]
PHST- 2006/06/20 09:00 [medline]
PHST- 2005/12/20 09:00 [entrez]
AID - 10.1002/mrd.20419 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2006 Mar;73(3):342-9. doi: 10.1002/mrd.20419.