PMID- 16363699
OWN - NLM
STAT- MEDLINE
DCOM- 20060310
LR  - 20110727
IS  - 0047-1852 (Print)
IS  - 0047-1852 (Linking)
VI  - 63
IP  - 12
DP  - 2005 Dec
TI  - [Liver regenerative therapy using glycoside-modified bone marrow].
PG  - 2229-36
AB  - Recent studies have reported that bone marrow cells (BMCs) have the ability to 
      generate functional hepatocytes. However, the efficiency with which BMC 
      transplantation generates functional hepatocytes is rather low. We assumed that 
      if BMCs accumulated directly in liver, the functional BMC-derived hepatocytes 
      should increase more efficiently. An attempt was made to increase the 
      accumulation of BMCs directly in liver through the interaction between hepatic 
      asialoglycoprotein receptor (ASGPR) and galactose-exposing BMCs. 
      Galactose-exposing BMCs that expressed green fluorescent protein (GFP) were 
      injected into Long-Evans-Cinnamon (LEC) rats, a Wilson's disease (WD) model, via 
      the tail vein. The WD is an autosomal-recessive disorder characterized by 
      impaired biliary copper excretion and copper toxicosis, all due to mutations in 
      the atp7b gene. At 5 months after transplantation, GFP-expressing hepatocyte 
      nodules accounted for 2.4% of total liver mass, and the normal ceruloplasmin was 
      detectable in the sera of these LEC rats. These findings suggest that the 
      functional BMC-derived hepatocytes can be generated and the new genes derived 
      from BMCs, such as ATP7B and GFP, can be transferred to LEC rats by the direct 
      accumulation of BMCs in liver without hematopoietic reconstitution in need of 
      preparative lethal irradiation.
FAU - Ise, Hirohiko
AU  - Ise H
AD  - Department of Organ Regeneration, Shinshu University Graduate School of Medicine.
FAU - Takahashi, Masafumi
AU  - Takahashi M
FAU - Ikeda, Uichi
AU  - Ikeda U
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Nihon Rinsho
JT  - Nihon rinsho. Japanese journal of clinical medicine
JID - 0420546
RN  - 0 (Asialoglycoprotein Receptor)
RN  - 0 (Glycosides)
SB  - IM
MH  - Animals
MH  - Asialoglycoprotein Receptor/physiology
MH  - Bone Marrow Transplantation/*methods
MH  - Glycosides/*metabolism
MH  - Liver Regeneration/*physiology
MH  - Mice
MH  - Rats
RF  - 19
EDAT- 2005/12/21 09:00
MHDA- 2006/03/11 09:00
CRDT- 2005/12/21 09:00
PHST- 2005/12/21 09:00 [pubmed]
PHST- 2006/03/11 09:00 [medline]
PHST- 2005/12/21 09:00 [entrez]
PST - ppublish
SO  - Nihon Rinsho. 2005 Dec;63(12):2229-36.