PMID- 16364153
OWN - NLM
STAT- MEDLINE
DCOM- 20060607
LR  - 20171116
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 61
IP  - 1
DP  - 2006 Jan
TI  - CD4+ CD30+ T cells perpetuate IL-5 production in Dermatophagoides pteronyssinus 
      allergic patients.
PG  - 27-34
AB  - BACKGROUND: Airway allergic diseases are regulated by interleukin (IL)-5, which 
      causes infiltration of eosinophils into the bronchial epithelium, and by IL-4 
      which increases serum immunoglobulin E (IgE) production and promotes CD30 
      expression on Th cells. CD30 generates a costimulatory signal involved in 
      apoptosis or cell proliferation, depending on the microenvironment. Our aims 
      were: (i) to analyze if CD4+ CD30+ T cells from allergic patients proliferate in 
      response to Dermatophagoides pteronyssinus, and (ii) if upon stimulation this 
      cell population produces IL-4 and IL-5. METHODS: Peripheral blood mononuclear 
      cell (PBMC) from 17 allergic rhinitis and mild allergic asthma patients and 12 
      healthy nonallergic individuals were stimulated with allergen in the presence or 
      absence of anti-IL-4, anti-IL-5 or anti-IL-4Ralpha monoclonal antibodies (mAbs). 
      TdT-mediated dUTP nick end-labeling (TUNEL) assay, 7-aminoactinomycin-D (7-AAD) 
      intercalation, and flow cytometry were used to determine the CD4+ CD30+ blasts 
      percentage, cell proliferation, apoptosis, and intracellular cytokines after 7 
      culture days. RESULTS: Cell proliferation induced with allergen showed that 90% 
      of the allergen-stimulated blasts were CD4+, 50% of which were CD30+. 
      Allergen-stimulated PBMC showed a progressive increase (mean: from 7% to 23%) of 
      CD4+ CD30+IFN-gamma+ and CD4+ CD30+IL-4+ blasts which diminished (mean: 6%) after 
      5 culture days. In contrast, CD4+ CD30+IL-5+ blasts showed a continuous 
      progression (from 12% to 24%) that maintained after 7 culture days. The vast 
      majority of CD4+ CD30+ blasts were negative to 7-AAD or TUNEL. Additionally, a 
      significant decrease (34%) was observed in the number of CD4+ CD30+ blasts when 
      IL-4 was neutralized. CONCLUSIONS: These data suggest that specific allergen 
      stimulation of PBMC isolated from allergic patients generates a nonapoptotic CD4+ 
      CD30+ blast subset that produces IL-5.
FAU - Garfias, Y
AU  - Garfias Y
AD  - Instituto de Oftalmologia, Fundacion Conde de Valenciana, Mexico.
FAU - Ortiz, B
AU  - Ortiz B
FAU - Hernandez, J
AU  - Hernandez J
FAU - Magana, D
AU  - Magana D
FAU - Becerril-Angeles, M
AU  - Becerril-Angeles M
FAU - Zenteno, E
AU  - Zenteno E
FAU - Lascurain, R
AU  - Lascurain R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Interleukin-5)
RN  - 0 (Ki-1 Antigen)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/*pharmacology
MH  - Antigens, Dermatophagoides/*immunology
MH  - Apoptosis/immunology
MH  - Asthma/blood
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Case-Control Studies
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - *Dermatophagoides pteronyssinus
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-5/*biosynthesis
MH  - Ki-1 Antigen/immunology
MH  - Lymphocyte Activation
MH  - Male
MH  - Probability
MH  - Rhinitis, Allergic, Seasonal/blood
MH  - Sampling Studies
MH  - Sensitivity and Specificity
MH  - Statistics, Nonparametric
EDAT- 2005/12/21 09:00
MHDA- 2006/06/08 09:00
CRDT- 2005/12/21 09:00
PHST- 2005/12/21 09:00 [pubmed]
PHST- 2006/06/08 09:00 [medline]
PHST- 2005/12/21 09:00 [entrez]
AID - ALL951 [pii]
AID - 10.1111/j.1398-9995.2005.00951.x [doi]
PST - ppublish
SO  - Allergy. 2006 Jan;61(1):27-34. doi: 10.1111/j.1398-9995.2005.00951.x.