PMID- 16364868
OWN - NLM
STAT- MEDLINE
DCOM- 20060707
LR  - 20220330
IS  - 1557-3117 (Electronic)
IS  - 1053-2498 (Linking)
VI  - 24
IP  - 12
DP  - 2005 Dec
TI  - Identification of male cardiomyocytes of extracardiac origin in the hearts of 
      women with male progeny: male fetal cell microchimerism of the heart.
PG  - 2179-83
AB  - BACKGROUND: Fetal progenitor cells may cross the placenta during pregnancy, 
      persist for decades in the maternal bloodstream, and find a microenvironment 
      conducive to colonization in a variety of maternal solid organs. Whether 
      extracardiac fetal progenitors are present in the heart of women with male issue 
      is unknown. METHODS: The hearts from 2 non-pregnant women who had given birth to 
      2 and 3 male children, respectively, were studied. Myocardial specimens from 2 
      men and 2 women (without history of pregnancies) were used as controls. Real time 
      polymerase chain reaction was performed to amplify the SRY gene located at the Y 
      chromosome. Fluorescence in situ hybridization (FISH) with probes specific for X 
      and Y chromosomes was combined with alpha-actin immunohistochemistry to identify 
      cardiac muscle cells. Histocompatibility studies were conducted in both patients 
      and their male relatives. RESULTS: The SRY gene was amplified in the myocardium 
      of both patients. FISH analysis showed clear evidence of male cells with the 
      typical cardiomyocyte phenotype within the myocardium. X- and Y-chromosome bodies 
      in the nuclei were found in 0.25% and 0.20% of cells, respectively. Increased 
      human leukocyte antigen compatibility was observed between patients and their 
      sons. CONCLUSIONS: This study identified male cardiomyocytes of extracardiac 
      origin, presumably fetal, in the hearts of 2 women with male progeny. Fetal 
      progenitor cells may colonize the heart and under appropriate microenvironmental 
      stimuli, differentiate into cardiomyocytes.
FAU - Bayes-Genis, Antoni
AU  - Bayes-Genis A
AD  - Servei de Cardiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. 
      abayesgenis@hsp.santpau.es
FAU - Bellosillo, Beatriz
AU  - Bellosillo B
FAU - de la Calle, Oscar
AU  - de la Calle O
FAU - Salido, Marta
AU  - Salido M
FAU - Roura, Santiago
AU  - Roura S
FAU - Ristol, Francesc Sole
AU  - Ristol FS
FAU - Soler, Carolina
AU  - Soler C
FAU - Martinez, Monica
AU  - Martinez M
FAU - Espinet, Blanca
AU  - Espinet B
FAU - Serrano, Sergi
AU  - Serrano S
FAU - Bayes de Luna, Antoni
AU  - Bayes de Luna A
FAU - Cinca, Juan
AU  - Cinca J
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20050928
PL  - United States
TA  - J Heart Lung Transplant
JT  - The Journal of heart and lung transplantation : the official publication of the 
      International Society for Heart Transplantation
JID - 9102703
SB  - IM
MH  - Cardiomyopathy, Dilated
MH  - Cell Differentiation
MH  - *Cell Movement
MH  - *Chimerism
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Female
MH  - Genes, sry
MH  - Heart Transplantation
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Maternal-Fetal Exchange
MH  - Middle Aged
MH  - Myocardium/*cytology
MH  - Myocytes, Cardiac/*physiology
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Stem Cells/*physiology
EDAT- 2005/12/21 09:00
MHDA- 2006/07/11 09:00
CRDT- 2005/12/21 09:00
PHST- 2005/01/31 00:00 [received]
PHST- 2005/05/16 00:00 [revised]
PHST- 2005/06/07 00:00 [accepted]
PHST- 2005/12/21 09:00 [pubmed]
PHST- 2006/07/11 09:00 [medline]
PHST- 2005/12/21 09:00 [entrez]
AID - S1053-2498(05)00378-5 [pii]
AID - 10.1016/j.healun.2005.06.003 [doi]
PST - ppublish
SO  - J Heart Lung Transplant. 2005 Dec;24(12):2179-83. doi: 
      10.1016/j.healun.2005.06.003. Epub 2005 Sep 28.