PMID- 163655
OWN - NLM
STAT- MEDLINE
DCOM- 19750609
LR  - 20191210
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 383
IP  - 3
DP  - 1975 Mar 21
TI  - A simple and inexpensive method for the preparation of (beta,gamma-32P) guanosine 
      5'-triphosphate.
PG  - 229-35
AB  - A rapid and inexpensive method has been developed for the synthesis of 
      32P-labeled guanosine 5'-triphosphate (GTP). When yolk platelets isolated from 
      brine shrimp cysts are incubated with 32PPi at pH 5.8 and in the presence of 10 
      mM MgCl2 and 5 mM dithiothreitol, the primary compound formed is 
      [beta,gamma-32P]GTP. The synthetic reaction is catalyzed by the yolk platelet 
      enzyme, GTP : GTP guanylyltransferase, which has been demonstrated to be 
      important in the biosynthesis of diguanosine 5'-tetraphosphate (Gp4G), the major 
      purine nucleotide in brine shrimp yolk platelets and encysted embryos.
FAU - Warner, A H
AU  - Warner AH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Diphosphates)
RN  - 0 (Ethanolamines)
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Phosphorus Radioisotopes)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Animals
MH  - Decapoda/embryology/*metabolism
MH  - Diphosphates/metabolism
MH  - Dithiothreitol/metabolism
MH  - Embryo, Nonmammalian/metabolism
MH  - Ethanolamines/metabolism
MH  - Guanine Nucleotides/metabolism
MH  - Guanosine Triphosphate/*biosynthesis/isolation & purification
MH  - Isotope Labeling
MH  - Nucleotidyltransferases/metabolism
MH  - *Phosphorus Radioisotopes
EDAT- 1975/03/21 00:00
MHDA- 1975/03/21 00:01
CRDT- 1975/03/21 00:00
PHST- 1975/03/21 00:00 [pubmed]
PHST- 1975/03/21 00:01 [medline]
PHST- 1975/03/21 00:00 [entrez]
AID - 0005-2787(75)90051-9 [pii]
PST - ppublish
SO  - Biochim Biophys Acta. 1975 Mar 21;383(3):229-35.