PMID- 1636677
OWN - NLM
STAT- MEDLINE
DCOM- 19920821
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 263
IP  - 1 Pt 1
DP  - 1992 Jul
TI  - Regulated expression of monocyte chemoattractant protein-1 in normal human 
      osteoblastic cells.
PG  - C194-9
AB  - An influx of monocytes is observed in many different physiological and 
      pathophysiological states, including bone remodeling and injury. These cells 
      appear at early stages of bone formation and repair and persist throughout the 
      later stages. In experiments described here, unstimulated normal human 
      osteoblastic cells did not produce detectable levels of monocyte chemotactic 
      activity. However, interleukin-1 (IL-1)-stimulated normal human osteoblastic 
      cells produced a chemoattractant that is similar to monocyte chemoattractant 
      protein-1 (MCP-1) at the levels of mRNA expression, protein production, and 
      chemotactic activity. Northern blot analysis indicates that IL-1 elicits a 
      dose-dependent increase in MCP-1 mRNA in normal human osteoblastic cells. Two 
      proteins of M(r) 9,000 and M(r) 13,000 were specifically immunoprecipitated with 
      MCP-1 antiserum from IL-1-stimulated normal human osteoblastic cells. Monocyte 
      chemotactic activity from IL-1-treated cells was blocked by MCP-1 antiserum. 
      These studies establish that normal human osteoblastic cells can be induced to 
      produce monocyte chemoattractants and that this is accounted for by the induced 
      expression of MCP-1.
FAU - Williams, S R
AU  - Williams SR
AD  - Department of Oral Biology, Boston University Medical Center, Massachusetts 
      02118.
FAU - Jiang, Y
AU  - Jiang Y
FAU - Cochran, D
AU  - Cochran D
FAU - Dorsam, G
AU  - Dorsam G
FAU - Graves, D T
AU  - Graves DT
LA  - eng
GR  - DE-07559/DE/NIDCR NIH HHS/United States
GR  - DE-08569/DE/NIDCR NIH HHS/United States
GR  - DE-09581/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-1)
SB  - IM
MH  - Blood Platelets/metabolism
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemotactic Factors/blood/*metabolism/pharmacology
MH  - Culture Media
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Macrophages/physiology
MH  - Monocytes/physiology
MH  - Osteoblasts/*metabolism/physiology
MH  - Reference Values
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
AID - 10.1152/ajpcell.1992.263.1.C194 [doi]
PST - ppublish
SO  - Am J Physiol. 1992 Jul;263(1 Pt 1):C194-9. doi: 10.1152/ajpcell.1992.263.1.C194.