PMID- 16367770
OWN - NLM
STAT- MEDLINE
DCOM- 20060302
LR  - 20131121
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 22
IP  - 12
DP  - 2005 Dec
TI  - Chondroitinase ABC combined with neural stem/progenitor cell transplantation 
      enhances graft cell migration and outgrowth of growth-associated 
      protein-43-positive fibers after rat spinal cord injury.
PG  - 3036-46
AB  - We previously reported that the transplantation of neural stem/progenitor cells 
      (NSPCs) can contribute to the repair of injured spinal cord in adult rats and 
      monkeys. In some cases, however, most of the transplanted cells adhered to the 
      cavity wall and failed to migrate and integrate into the host spinal cord. In 
      this study we focused on chondroitin sulfate proteoglycan (CSPG), a known 
      constituent of glial scars that is strongly expressed after spinal cord injury 
      (SCI), as a putative inhibitor of NSPC migration in vivo. We hypothesized that 
      the digestion of CSPG by chondroitinase ABC (C-ABC) might promote the migration 
      of transplanted cells and neurite outgrowth after SCI. An in vitro study revealed 
      that the migration of NSPC-derived cells was inhibited by CSPG and that this 
      inhibitory effect was attenuated by C-ABC pre-treatment. Consistently, an in vivo 
      study of C-ABC treatment combined with NSPC transplantation into injured spinal 
      cord revealed that C-ABC pre-treatment promoted the migration of the transplanted 
      cells, whereas CSPG-immunopositive scar tissue around the lesion cavity prevented 
      their migration into the host spinal cord in the absence of C-ABC pre-treatment. 
      Furthermore, this combined treatment significantly induced the outgrowth of a 
      greater number of growth-associated protein-43-positive fibers at the lesion 
      epicentre, compared with NSPC transplantation alone. These findings suggested 
      that the application of C-ABC enhanced the benefits of NSPC transplantation for 
      SCI by reducing the inhibitory effects of the glial scar, indicating that this 
      combined treatment may be a promising strategy for the regeneration of injured 
      spinal cord.
FAU - Ikegami, Takeshi
AU  - Ikegami T
AD  - Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, 
      Japan.
FAU - Nakamura, Masaya
AU  - Nakamura M
FAU - Yamane, Junichi
AU  - Yamane J
FAU - Katoh, Hiroyuki
AU  - Katoh H
FAU - Okada, Seiji
AU  - Okada S
FAU - Iwanami, Akio
AU  - Iwanami A
FAU - Watanabe, Kota
AU  - Watanabe K
FAU - Ishii, Ken
AU  - Ishii K
FAU - Kato, Fumikazu
AU  - Kato F
FAU - Fujita, Hiroshi
AU  - Fujita H
FAU - Takahashi, Toyomi
AU  - Takahashi T
FAU - Okano, Hirotaka James
AU  - Okano HJ
FAU - Toyama, Yoshiaki
AU  - Toyama Y
FAU - Okano, Hideyuki
AU  - Okano H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nes protein, rat)
RN  - 0 (Nestin)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Tubulin)
RN  - EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - 2',3'-Cyclic-Nucleotide Phosphodiesterases
MH  - Analysis of Variance
MH  - Animals
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Movement/*drug effects
MH  - Cell Survival/drug effects
MH  - Chondroitin ABC Lyase/*pharmacology
MH  - Chondroitin Sulfate Proteoglycans/metabolism/pharmacology
MH  - Chromatography, High Pressure Liquid/methods
MH  - Disease Models, Animal
MH  - Drug Interactions
MH  - GAP-43 Protein/metabolism
MH  - Gene Expression Regulation/physiology
MH  - Glial Fibrillary Acidic Protein
MH  - Immunohistochemistry/methods
MH  - Intermediate Filament Proteins/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Nestin
MH  - Neurofilament Proteins
MH  - Neurons/*drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/*surgery
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/*drug effects/physiology
MH  - Time Factors
MH  - Tubulin
EDAT- 2005/12/22 09:00
MHDA- 2006/03/03 09:00
CRDT- 2005/12/22 09:00
PHST- 2005/12/22 09:00 [pubmed]
PHST- 2006/03/03 09:00 [medline]
PHST- 2005/12/22 09:00 [entrez]
AID - EJN4492 [pii]
AID - 10.1111/j.1460-9568.2005.04492.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2005 Dec;22(12):3036-46. doi: 10.1111/j.1460-9568.2005.04492.x.