PMID- 16368891
OWN - NLM
STAT- MEDLINE
DCOM- 20060508
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 107
IP  - 7
DP  - 2006 Apr 1
TI  - A transforming growth factor-beta-induced protein stimulates endocytosis and is 
      up-regulated in immature dendritic cells.
PG  - 2777-85
AB  - Dendritic cells (DCs) exhibit distinct functional properties at immature and 
      mature states. To identify genes preferentially regulated in monocyte-derived 
      immature DCs (imDCs), 13 000-element microarrays were hybridized with RNA 
      isolated from imDCs, mature DCs (mDCs), monocytes, and macrophages and a 
      TGF-beta-induced protein (betaig-h3) was identified as being most prominently 
      up-regulated in imDCs. By polymerase chain reaction (PCR), little betaig-h3 mRNA 
      was detected in monocytes and macrophages, but it was abundant in imDCs. On DC 
      activation with LPS, betaig-h3 mRNA became diminished, and in tissues, betaig-h3 
      mRNA was abundantly expressed in lymphoid-rich tissues such as the spleen, bone 
      marrow, small intestines, and colon. betaig-h3 was expressed in 293T cells and 
      purified as a 70-kDa protein and, by Western blotting, betaig-h3 was 
      predominantly detected in the medium of imDCs. We demonstrate that betaig-h3 
      binds to macrophages and imDCs but not to mDCs and activates the Rac GTPase in 
      macrophages, stimulating macrophage membrane ruffling and enhancing macrophage 
      endocytosis. imDC endocytosis was also inhibited by purified anti-betaig-h3 
      antibodies. Therefore, betaig-h3 appears to be selectively up-regulated in imDCs 
      to regulate antigen uptake through endocytosis.
FAU - Cao, Weiping
AU  - Cao W
AD  - Department of Microbiology, Yong Loo Lin School of Medicine, National University 
      of Singapore, Blk MD4, 5 Science Dr 2, Singapore 117597.
FAU - Tan, Patrick
AU  - Tan P
FAU - Lee, Chee How
AU  - Lee CH
FAU - Zhang, Haifeng
AU  - Zhang H
FAU - Lu, Jinhua
AU  - Lu J
LA  - eng
PT  - Journal Article
DEP - 20051220
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Complementary)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 148710-76-3 (betaIG-H3 protein)
SB  - IM
MH  - Cell Membrane/physiology/ultrastructure
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Dendritic Cells/*immunology
MH  - Endocytosis/*immunology
MH  - Extracellular Matrix Proteins/*genetics/immunology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Macrophages/immunology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction
MH  - Transforming Growth Factor beta/genetics/immunology/*physiology
EDAT- 2005/12/22 09:00
MHDA- 2006/05/09 09:00
CRDT- 2005/12/22 09:00
PHST- 2005/12/22 09:00 [pubmed]
PHST- 2006/05/09 09:00 [medline]
PHST- 2005/12/22 09:00 [entrez]
AID - S0006-4971(20)65616-6 [pii]
AID - 10.1182/blood-2005-05-1803 [doi]
PST - ppublish
SO  - Blood. 2006 Apr 1;107(7):2777-85. doi: 10.1182/blood-2005-05-1803. Epub 2005 Dec 
      20.