PMID- 16371718
OWN - NLM
STAT- MEDLINE
DCOM- 20060314
LR  - 20171116
IS  - 1598-9992 (Print)
IS  - 1598-9992 (Linking)
VI  - 46
IP  - 6
DP  - 2005 Dec
TI  - [Effects of TNF-alpha on angiogenesis in CT-26 mouse colon carcinoma homograft 
      model].
PG  - 440-6
AB  - BACKGROUND/AIMS: Tumor necrosis factor-alpha (TNF-alpha) exerts its anti-tumor 
      effect through direct cytotoxicity on tumor cells and damage to the tumor 
      vasculature. However, its role in tumor angiogenesis is controversial. We 
      evaluated the angiogenic effect of TNF-alpha on BALB/c mouse colon carcinoma 
      homograft model. METHODS: Ten BALB/c mice were inoculated intraperitoneally with 
      CT-26 mouse colon carcinoma cells. After a week, recombinant mouse TNF-alpha (2 
      microg/mL) were given four times on every other day to five animals and the same 
      volume of phosphate buffered saline was given at the same interval to five 
      animals as control. Harvested tumor tissues were stained by immunohistochemistry 
      with CD31 and VEGF antibodies. Number of microvessels and VEGF expression were 
      counted by light microscope. RESULTS: The mean microvessel counts per 200x field 
      of TNF-alpha treated animals were 70.2+/-7.8 and those of nontreated animals were 
      83.8+/-8.3 (p<0.05). The VEGF score of both groups were 3. CONCLUSIONS: TNF-alpha 
      treated animals showed decreased microvessel counts in tumor tissue but VEGF 
      expression in both groups showed no difference. Therefore, TNF-alpha showed 
      antiangiogenic effects on colon carcinoma homograft model.
FAU - Chun, Jong Woon
AU  - Chun JW
AD  - Departments of Internal Medicine, Catholic University of Daegu School of 
      Medicine, Daegu, Korea.
FAU - Jung, Jin Tae
AU  - Jung JT
FAU - Oh, Hoon Kyu
AU  - Oh HK
FAU - Bae, Jong Yup
AU  - Bae JY
FAU - Shin, Im Hee
AU  - Shin IH
FAU - Cho, Chang Ho
AU  - Cho CH
LA  - kor
PT  - Journal Article
PL  - Korea (South)
TA  - Korean J Gastroenterol
JT  - The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi
JID - 101189416
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Carcinoma/chemistry/*physiopathology
MH  - Cell Line, Tumor
MH  - Colonic Neoplasms/chemistry/*physiopathology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neovascularization, Pathologic/*physiopathology
MH  - Platelet Endothelial Cell Adhesion Molecule-1/analysis
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Vascular Endothelial Growth Factor A/analysis
EDAT- 2005/12/24 09:00
MHDA- 2006/03/15 09:00
CRDT- 2005/12/24 09:00
PHST- 2005/12/24 09:00 [pubmed]
PHST- 2006/03/15 09:00 [medline]
PHST- 2005/12/24 09:00 [entrez]
AID - 200512254 [pii]
PST - ppublish
SO  - Korean J Gastroenterol. 2005 Dec;46(6):440-6.