PMID- 16372582
OWN - NLM
STAT- MEDLINE
DCOM- 20060126
LR  - 20170214
IS  - 0300-0605 (Print)
IS  - 0300-0605 (Linking)
VI  - 33
IP  - 6
DP  - 2005 Nov-Dec
TI  - DNA vaccine against hamster oral papillomavirus-associated oral cancer.
PG  - 647-53
AB  - Previously we developed a carcinogenesis model involving the combination of 
      9,10-dimethyl-1,2-benzanthracene (DMBA) application with physical wounding of 
      hamster lingual mucosa. The presence of a novel hamster oral papillomavirus 
      (HOPV) was demonstrated and its genome sequenced. In the present study, this HOPV 
      hamster model was used to test whether vaccination with the L1 gene could prevent 
      the development of oral carcinoma. DNA plasmids encoding the L1 gene or the 
      vector alone were injected intramuscularly into 20 vaccinated and 20 control 
      hamsters, respectively. The lingual tips of the hamsters were painted with DMBA 
      for 8 weeks. A portion of the lingual tips was excised, and the tips were then 
      painted daily with DMBA until the animals were killed 13 days later. All control 
      hamsters developed lingual carcinoma, whereas 12 of the L1-vaccinated hamsters 
      showed no lesions. These results suggest that immunization with L1 DNA vaccines 
      may prevent the development of papillomavirus-associated oral cancer.
FAU - Maeda, H
AU  - Maeda H
AD  - Department of Pathology, School of Dentistry, Aichi-Gakuin University, Nagoya, 
      Japan. hatsu@dpc.aichi-gakuin.ac.jp
FAU - Kubo, K
AU  - Kubo K
FAU - Sugita, Y
AU  - Sugita Y
FAU - Miyamoto, Y
AU  - Miyamoto Y
FAU - Komatsu, S
AU  - Komatsu S
FAU - Takeuchi, S
AU  - Takeuchi S
FAU - Umebayashi, T
AU  - Umebayashi T
FAU - Morikawa, S
AU  - Morikawa S
FAU - Kawanishi, K
AU  - Kawanishi K
FAU - Kameyama, Y
AU  - Kameyama Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Int Med Res
JT  - The Journal of international medical research
JID - 0346411
RN  - 0 (Capsid Proteins)
RN  - 0 (HPV L1 protein, Human papillomavirus)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Vaccines, DNA)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene/toxicity
MH  - Animals
MH  - Capsid Proteins
MH  - Cricetinae
MH  - Disease Models, Animal
MH  - Male
MH  - Mesocricetus
MH  - Mouth Neoplasms/chemically induced/pathology/*prevention & control
MH  - Oncogene Proteins, Viral/genetics
MH  - Papillomavirus Infections/complications/pathology/*prevention & control
MH  - Vaccines, DNA/genetics/*pharmacology
EDAT- 2005/12/24 09:00
MHDA- 2006/01/27 09:00
CRDT- 2005/12/24 09:00
PHST- 2005/12/24 09:00 [pubmed]
PHST- 2006/01/27 09:00 [medline]
PHST- 2005/12/24 09:00 [entrez]
AID - 10.1177/147323000503300606 [doi]
PST - ppublish
SO  - J Int Med Res. 2005 Nov-Dec;33(6):647-53. doi: 10.1177/147323000503300606.