PMID- 16374466
OWN - NLM
STAT- MEDLINE
DCOM- 20060307
LR  - 20171116
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 126
IP  - 2
DP  - 2006 Feb
TI  - Presence of chimeric maternally derived keratinocytes in cutaneous inflammatory 
      diseases of children: the example of pityriasis lichenoides.
PG  - 345-8
AB  - During pregnancy, maternal cells may enter the fetal circulation and persist 
      until adulthood. The fate of these cells remains unknown. As unexplained 
      T-cell-mediated conditions such as pityriasis lichenoides (PL) may occur in 
      children, we aimed at identifying maternal cells in lesional skin of PL and 
      controls. Archived skin biopsy specimens from young males with PL, atopic 
      dermatitis, or normal skin were scanned for the presence of female (presumably 
      maternal) cells using fluorescence in situ hybridization (FISH) with X and Y 
      chromosome-specific probes. Phenotyping of maternal cells relied on FISH combined 
      with anti-CD45, anti-CD1a, or anti-cytokeratin labelling, identifying leukocytes, 
      Langerhans cells, and keratinocytes, respectively. Maternal cells were found in 
      PL (11/12) and controls (4/7), but their average frequency was higher in PL: 99 
      per million cells as compared to 5 per million cells in controls (P = 0.005). In 
      the epidermis, the maternal microchimeric cells were labelled by anti-cytokeratin 
      in all cases. We identified maternally derived keratinocytes in the skin of male 
      children with inflammatory skin disorders. These cells may either help repair the 
      damaged skin or home initially in the skin and trigger a host (child) versus 
      graft (mother) disease.
FAU - Khosrotehrani, Kiarash
AU  - Khosrotehrani K
AD  - Service de dermatologie, Hopital Tenon, Paris, France.
FAU - Guegan, Sarah
AU  - Guegan S
FAU - Fraitag, Sylvie
AU  - Fraitag S
FAU - Oster, Michele
AU  - Oster M
FAU - de Prost, Yves
AU  - de Prost Y
FAU - Bodemer, Christine
AU  - Bodemer C
FAU - Aractingi, Selim
AU  - Aractingi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 68238-35-7 (Keratins)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
CIN - J Invest Dermatol. 2006 Feb;126(2):239-41. PMID: 16418731
MH  - Antigens, CD1/analysis
MH  - Child
MH  - Child, Preschool
MH  - *Chimera
MH  - Chimerism
MH  - Dermatitis, Atopic/genetics/*pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratinocytes/chemistry/*pathology
MH  - Keratins/analysis
MH  - Leukocyte Common Antigens/analysis
MH  - Male
MH  - *Maternal-Fetal Exchange
MH  - Pityriasis Lichenoides/genetics/*pathology
MH  - Pregnancy
EDAT- 2005/12/24 09:00
MHDA- 2006/03/08 09:00
CRDT- 2005/12/24 09:00
PHST- 2005/12/24 09:00 [pubmed]
PHST- 2006/03/08 09:00 [medline]
PHST- 2005/12/24 09:00 [entrez]
AID - S0022-202X(15)32747-0 [pii]
AID - 10.1038/sj.jid.5700060 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2006 Feb;126(2):345-8. doi: 10.1038/sj.jid.5700060.