PMID- 16374897
OWN - NLM
STAT- MEDLINE
DCOM- 20060425
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 26
IP  - 1
DP  - 2006 Jan
TI  - Quantification of fetal nucleated cells in maternal blood of pregnant women with 
      a male trisomy 21 fetus using molecular cytogenetic techniques.
PG  - 28-34
AB  - BACKGROUND: Prenatal diagnosis of trisomy 21 is based on fetal karyotyping 
      generally obtained using invasive methods. During pregnancy, the circulating 
      fetal cells in maternal blood constitute a potential source for development of a 
      noninvasive prenatal diagnosis. The objective of this study was the 
      identification and quantification of all fetal nucleated cells per unit volume of 
      peripheral blood of pregnant women carrying male fetuses with trisomy 21 using 
      molecular cytogenetic techniques. METHODS: Peripheral blood samples were obtained 
      from 16 women carrying male fetuses with trisomy 21. We used a simple and rapid 
      method of harvesting blood without recourse to any enrichment procedures or 
      cell-separation techniques. To evaluate the potential of this method, 16 
      specimens were analyzed by molecular cytogenetic techniques such as fluorescence 
      in situ hybridization (FISH) and primed in situ labeling (PRINS) using specific 
      probes to chromosomes X, Y and 21. RESULTS: The number of fetal cells varied 
      between 6 and 32 per mL of maternal blood. This number is 3-5 times higher than 
      that from normal pregnancies. CONCLUSIONS: Our current results are in agreement 
      with the results previously reported by other groups showing that the number of 
      fetal cells in maternal blood in trisomic 21 pregnancies is higher than in normal 
      pregnancies. This high number of fetal cells is regarded as an advantage for the 
      development of a noninvasive prenatal diagnostic test.
CI  - 2006 John Wiley & Sons, Ltd.
FAU - Krabchi, Kada
AU  - Krabchi K
AD  - Service of Genetics, Department of Pediatrics, Faculty of Medicine and Health 
      Sciences, University of Sherbrooke, Sherbrooke, Quebec, Canada.
FAU - Gadji, Macoura
AU  - Gadji M
FAU - Samassekou, Oumar
AU  - Samassekou O
FAU - Gregoire, Marie-Chantal
AU  - Gregoire MC
FAU - Forest, Jean-Claude
AU  - Forest JC
FAU - Drouin, Regen
AU  - Drouin R
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - Chromosomes, Human, Pair 21/chemistry
MH  - Chromosomes, Human, X/chemistry
MH  - Chromosomes, Human, Y/chemistry
MH  - *Cytogenetic Analysis
MH  - Down Syndrome/blood/*diagnosis
MH  - Erythroblasts/*chemistry
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Predictive Value of Tests
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Primed In Situ Labeling
MH  - Sensitivity and Specificity
EDAT- 2005/12/24 09:00
MHDA- 2006/04/28 09:00
CRDT- 2005/12/24 09:00
PHST- 2005/12/24 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2005/12/24 09:00 [entrez]
AID - 10.1002/pd.1325 [doi]
PST - ppublish
SO  - Prenat Diagn. 2006 Jan;26(1):28-34. doi: 10.1002/pd.1325.